Novel stationary phases based on epoxidized polystyrene-divinylbenzene for three modes of liquid chromatography

Abstract

Mixed-mode stationary phases based on epoxidized copolymer of styrene and divinylbenzene have been obtained by amination with methylamine, further alkylation with 1,4-butanediol diglycidyl ether and opening the terminal oxirane rings with dimethylethanolamine. To evaluate the effect of the number of anchor amino and diol groups on the degree of hydrophilization of resins, the quantity of reagents was varied. Polymerization of glycidol in the functional layer at an increased pH of the reaction medium was carried out for additional shielding of the substrate. It was found that increasing the number of anchor amino groups is promising for increasing hydrophilicity in suppressed ion chromatography and hydrophilic interaction liquid chromatography modes, while polymerization of glycidol increases the degree of substrate shielding. The applicability of the most hydrophilic adsorbent in three HPLC modes was demonstrated. Novel mixed-mode stationary phase allows the separation of six nucleosides and nitrogenous bases by hydrophilic interaction liquid chromatography, 7 alkylbenzenes by reversed phase liquid chromatography, and 20 organic and inorganic anions by suppressed ion chromatography.