Study on some biological characteristics of the albino strain of medicinal fungus Cordyceps militaris isolated in Vietnam

Thi Kim Trang Do, Ngoc Anh Dao, Bao Tram Tran, Thi Chien Truong, Minh Nam Nguyen, Van Tuan Tran, Xuan Tao Vu
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Abstract

Cordyceps militaris is one of the important medicinal fungi in traditional medicine. The fruiting body of C. militaris contains various bioactive compounds that have health-enhancing effects and support the treatment of various diseases. Therefore, this fungus is a potential source of medicinal materials for the development of healthcare products. The search for new strains of C. militaris with high yield, quality, and uniqueness is always of great interest. This study has provided new findings on some biological characteristics of an albino strain of C. militaris isolated in Vietnam. The albino strain of C. militaris SHBTQ was isolated and accurately identified based on morphological characteristics and the ITS (internal transcribed spacer) sequence of ribosomal DNA. The mycelial of C. militaris SHBTQ is white and remains stable for five successive generations. The most suitable carbon and nitrogen sources for the growth of the albino SHBTQ strain were sucrose and yeast extract, respectively. Notably, the SHBTQ strain is capable of forming fruiting bodies, with an average fresh fruiting body yield of 30 g/box, a biological efficiency of 12.2%, and a cordycepin content of 10.45 mg/g of dried fruiting bodies. This newly discovered strain offers great potential for further applications in production.
越南产药用真菌蛹虫草白化菌株的生物学特性研究
蛹虫草是传统医学中重要的药用真菌之一。蚕豆子实体含有多种生物活性化合物,具有促进健康和支持治疗多种疾病的作用。因此,这种真菌是开发保健产品的潜在药材来源。寻找高产、优质、独特的军孢杆菌新品种一直是人们关注的焦点。本研究对越南分离的一株白化菌的一些生物学特性有了新的发现。利用形态学特征和核糖体DNA ITS (internal转录间隔物)序列,分离得到一株白化菌militaris SHBTQ。SHBTQ的菌丝呈白色,连续5代保持稳定。蔗糖和酵母浸膏分别是最适合白化菌SHBTQ生长的碳源和氮源。值得注意的是,SHBTQ菌株能够形成子实体,其平均鲜子实体产量为30 g/箱,生物效率为12.2%,干子实体中虫草素含量为10.45 mg/g。这种新发现的菌株在进一步的生产应用中具有很大的潜力。
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