Phytoligands analysis of aqueous seed extract of Momordica charantia Linn. via GC-MS for its antidiabetic activity

Q4 Earth and Planetary Sciences
Manju Bala, Anju Dhiman, Munish Garg, Harish Dureja
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引用次数: 0

Abstract

In India, Momordica charantia Linn. is utilized in juices, health supplements and colouring agents. The aim of the current study is to analyze phytoligands by utilizing gas chromatography mass spectroscopy of aqueous seed extract of Momordica charantia Linn (MCA). The MCA was prepared by macerating the powdered seeds for 72 hrs followed by filtration and lyophilization followed by an in vitro α-glucosidase inhibitory activity. MCA was the potent inhibitor of α-glucosidase with IC50 value 73.75± 0.05 μg/ml. The analysis through GC-MS investigated 60 phytoconstituents which were validated using Schrodinger suite version 2020-3 against α-glucosidase (PDB ID- 5NN5). As per docking results, the existing phytochemicals azetidin-2-one 3,3-dimethyl-4-(1-aminoethyl)-, alpha-l-rhamnopyranose, piperidine, 3-methyl-, 1,4-Dioxane-2,5-dione,3,6-dimethyl and citronellol epoxide (R or S) from MCA, got the highest d-score of -7.230, -5.918, -5.643, -5.312 and -5.185 kcal/mol and could be promising antidiabetic drug candidates.
苦瓜种子水提物的植物配体分析。通过GC-MS检测其抗糖尿病活性
在印度,Momordica charantia Linn。用于果汁、保健品和着色剂。采用气相色谱质谱法对苦瓜种子水提物中的植物配体进行分析。将种子粉末浸泡72小时,过滤、冻干,体外抑制α-葡萄糖苷酶活性,制备MCA。MCA是α-葡萄糖苷酶的有效抑制剂,IC50值为73.75±0.05 μg/ml。采用气相色谱-质谱法对60种植物成分进行了分析,并利用Schrodinger suite version 2020-3对α-葡萄糖苷酶(PDB ID- 5NN5)进行了验证。对接结果显示,现有植物化学物质azetitin -2- 1,3 -二甲基-4-(1-氨基乙基)-、α -l-鼠李糖、胡椒碱、3-甲基-、1,4-二氧嘧啶-2,5-二酮、3,6-二甲基和环氧香茅醇(R或S)的d值最高,分别为-7.230、-5.918、-5.643、-5.312和-5.185 kcal/mol,有望成为抗糖尿病的候选药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
0.50
自引率
0.00%
发文量
195
审稿时长
4-8 weeks
期刊介绍: Information not localized
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