Comparative Analysis of the Transcriptome and Metabolome in Leaves of Diploid and Tetraploid Fagopyrum tataricum

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Xiaodong Shi, Yue Qi, Liangzhu Lin, Jia Wang, Xiaobo Qin, Bei Niu
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引用次数: 0

Abstract

Tartary buckwheat (Fagopyrum tataricum) is a dual-purpose medicinal and food crop grown for its high contents of functional compounds and abundant nutrients. Although studies have shown the differences of total flavonoid content in Tartary buckwheat at different ploidy levels, the composition of flavonoid and its regulatory mechanisms are largely unknown. In this study, the leaf metabolome and transcriptome of diploid and tetraploid accessions of Tartary buckwheat were analyzed to gain insight into the impact of polyploidization on comparative secondary metabolite composition and molecular regulatory mechanism. Based on a widely targeted metabolomics analysis, a total of 792 metabolites were identified, including 127 flavonoids. The accumulation of 127 metabolites and expression of 3871 genes differed significantly between diploid and tetraploid Tartary buckwheat. Integrated metabolomics and transcriptome analysis revealed that chromosome doubling up-regulated the expression of upstream genes in the flavonoid biosynthesis pathway to promote the accumulation of flavonoids. The present results contribute to elucidation of the molecular mechanism of phenotypic variation associated with polyploidy in Tartary buckwheat. The findings provide a reference for further studies on phenotypic traits in polyploid Tartary buckwheat, the cloning of crucial regulatory genes, and utilization of genetic engineering technologies in the breeding of Tartary buckwheat.
二倍体和四倍体荞麦叶片转录组和代谢组的比较分析
苦荞(Fagopyrum tataricum)是一种药用和粮食两用作物,其功能化合物含量高,营养成分丰富。虽然研究表明不同倍性水平的苦荞总黄酮含量存在差异,但对其组成及其调控机制仍知之甚少。本研究通过对苦荞二倍体和四倍体材料的叶片代谢组和转录组进行分析,探讨多倍体化对苦荞相对次生代谢物组成的影响及其分子调控机制。基于广泛靶向的代谢组学分析,共鉴定出792种代谢物,其中包括127种黄酮类化合物。二倍体苦荞和四倍体苦荞127种代谢物的积累和3871个基因的表达差异显著。综合代谢组学和转录组学分析表明,染色体加倍上调黄酮类生物合成途径上游基因的表达,促进黄酮类物质的积累。本研究结果有助于阐明苦荞多倍体相关表型变异的分子机制。该研究结果为进一步研究多倍体苦荞的表型性状、关键调控基因的克隆以及基因工程技术在苦荞育种中的应用提供了参考。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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