Antioxidant Activity of Ethyl Acetate Fraction of Morinda citrifolia in Caenorhabditis elegans

Q4 Pharmacology, Toxicology and Pharmaceutics

Korean Journal of Pharmacognosy Pub Date : 2023-09-30 DOI:10.22889/kjp.2023.54.3.112

Ji Su Mun, Hyeoun ji Kim, Hyang Im Beak, Sang-Yong Ma, Dae Keun Kim, Suk Heung Oh, Jun Hyeong Kim

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Abstract

The ethanol extract of Morinda citrifolia was studied for antioxidative activity using a Caenorhabditis elegans model system. Ethyl acetate fraction of the ethyl alcohol extract showed the strongest DPPH, ABTS, superoxide radical scavenging activity. SOD and catalase enzymes, which confirm antioxidant enzyme activity in Caenorhabditis elegans, and reactive oxygen species (ROS), which can confirm the degree of oxidative stress resistance, were tested using the ethyl acetate fractions. Furthermore, in order to verify if regulation of stress response gene is responsible for the increased stress resistance of C. elegans which treated by the ethyl acetate fraction, we checked SOD-3 expression using a transgenic strain. Consequently, the ethyl acetate fraction of Morinda citrifolia Linn. increased the SOD and catalase activities in concentration dependent way in C. elegans, reduced ROS accumulation dose dependently. Besides, the ethyl acetate fraction-treated CF1553 worms showed more higher SOD-3::GFP intensity than the control group.

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桑椹叶乙酸乙酯部位对秀丽隐杆线虫的抗氧化活性研究

采用秀丽隐杆线虫模型系统研究了桑椹乙醇提取物的抗氧化活性。乙酸乙酯部位对DPPH、ABTS、超氧自由基的清除活性最强。采用乙酸乙酯组分测定了秀丽隐杆线虫抗氧化酶活性的超氧化物歧化酶(SOD)和过氧化氢酶(过氧化氢酶)，以及抗氧化应激程度的活性氧(ROS)。此外，为了验证应激反应基因的调控是否对经乙酸乙酯部分处理的秀丽隐杆线虫抗逆性的增强起作用，我们使用转基因菌株检测了SOD-3的表达。因此，桑葚的乙酸乙酯部分。提高线虫体内SOD和过氧化氢酶活性呈浓度依赖关系，降低ROS积累呈剂量依赖关系。此外，乙酸乙酯部分处理的CF1553虫SOD-3::GFP强度高于对照组。

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来源期刊

Korean Journal of Pharmacognosy Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

0.70

自引率

0.00%

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