Ameliorative effects of Tinospora cordifolia against cytogenotoxicity induced by lead acetate in Mus musculus

Abstract

Studies concerning exposure of humans to lead (Pb) during daily activities are sometimes leading to many complications. Pb is a poisonous heavy metal; its toxicity can change the antioxidant balance in biological systems. T. cordifolia is rich in phenolic acids and flavonoids which exhibit a wide range of biological effects and act as a natural antioxidant. The objective of the research work- first is to investigate the genotoxic potential of Pb-acetate on Bone marrow cells (BMCs), Second is to investigate the antigenotoxic potential of T. cordifolia against genotoxic induced by Pb-acetate in BMCs of Mus musculus (Swiss albino mice). For the research experiment, Adult Mus musculus of the same age group were selected andwere distributed into four groups (Group I, II, III &IV). After the completion of dose treatment, the experimental animals were euthanized by cervical dislocation and exposed both femora for the analysis of Micronucleus (Mn) in Polychromatic and Normochromatic erythrocytes. For the assessment of frequency of Mn in polychromatic or normochromatic erythrocytes 800 - 1000 cells were screened randomly under the compound microscope at 40X. and screening was done by image J microscopy software. Results of the experiment showed that the total frequency of Mn in polychromatic and normochromatic erythrocytes was 0.27 ± 0.07 in Group I, 0.18 ± 0.06 in Group II, 4.32 ± 0.32in Group III, and 0.94 ± 0.15 in Group IV and it is a significant difference to control and treated group at p < 0.05 or < 0.01. Hence, the results of the research experiment indicate that T. cordifolia stem aqueous extract has the potency to ameliorate and minimize the genotoxic induced by Pb-acetate in M. musculus (Swiss albino mice).