Gene expression pattern of K transporter GhHAK5 gene of potassium efficient and in-efficient cotton cultivars based on morphological physiognomies as affected by potassium nutrition and reduced irrigation

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Muhammad Naeem Akhtar, Tanveer ul Haq, Muhammad Waseem Akhtar, Ghulam Abbass
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Abstract

Abstract Background Under K deficiency the uptake and distribution pattern in plant cells is mediated through different transport proteins and channels which were controlled by specific gene family. Therefore, a hydroponic experiment was conducted under control condition for testing the gene expression pattern of the K transporter under adequate and low K supply levels. After that, a 2-year field experiment was conducted to evaluate five selected cotton cultivars (four K-efficient cultivars, viz., CIM-554, CYTO-124, FH-142, IUB-2013, and one K non-efficient, BH-212) screened from the initial hydroponics culture experiment and two levels of potassium (0 K 2 O kg·ha −1 and 50 K 2 O kg·ha −1 ) were tested under reduced irrigation (50% available water content; 50 AWC) and normal irrigation conditions (100% available water content; 100 AWC). Result Results revealed that the transcript levels of GhHAK5aD in roots were significantly higher in K + efficient cultivars than that in K + non-efficient cultivars. The GhHAK5aD expression upon K + deficiency was higher in roots but lower in shoots, indicating that GhHAK5aD could have a role in K + uptake in roots, instead of transport of K + from root to shoot. Similarly, under field conditions the cultivar FH-142 showed an increase of 22.3%, 4.9%, 2.4%, and 1.4% as compared with BH-212, IUB-2013, CYTO-124, and CIM-554, respectively, in seed cotton yield (SCY) with K application under reduced irrigation conditions. With applied K, the FH-142 showed an increase in net photosynthetic rate by 57.3% as compared with the rest of the cultivars under reduced irrigation over K control. However, the overall performance indicators of K-efficient cultivars like FH-142, CYTO-124, CIM-554, and IUB-2013 were better than BH-212 (K in-efficient) under reduced irrigation conditions with applied K at 50 kg·ha −1 . Fiber quality trait improved significantly with K application under water deficit. The increase in micronaire was 3.6%, 4.7%, 7.8%, 3.4%, and 6.7% in BH-212, IUB-2013, CIM-554, CYTO-124, and FH-142, respectively, with K application at 50 kg·ha −1 over without K application under reduced irrigation conditions during the cotton growing season. Similarly, the cultivars FH-142 increased by 12% with K application under reduced irrigation as compared with other cultivars. The performance of K-efficient cultivars under reduced irrigation conditions was 30% better in SCY and quality traits with the application of K at 50 kg·ha −1 as compared with K-non-efficient cultivars. Similarly, water use efficiency (WUE) (40.1%) and potassium use efficiency (KUE) (20.2%) were also noted higher in case of FH-142 as compared with other cultivar with K application under reduced conditions. Conclusion Higher expression of GhHAK5aD gene was observed in K-efficient cultivars as compared with K-non-efficient cultivars in roots indicates that GhHAK5aD may be contributing to genotypic differences for K + efficiency in cotton. K-efficient cotton cultivars can be used for the low-K environments and can also be recommended for general cultivars.
钾营养和减少灌溉对钾高效棉和低效棉K转运体GhHAK5基因形态特征的影响
背景在缺钾条件下,植物细胞对钾的吸收和分配模式是通过不同的转运蛋白和通道介导的,这些转运蛋白和通道受特定基因家族的控制。因此,在对照条件下进行了水培试验,以检测钾供应充足和低钾水平下钾转运体的基因表达模式。随后,通过为期2年的田间试验,对从初始水培试验中筛选出的5个棉花品种(4个钾肥高效品种:cmm -554、CYTO-124、FH-142、IUB-2013和1个钾肥低效品种:BH-212)进行了评价,并在减少灌溉(50%有效含水量;50 AWC)和正常灌溉条件(100%有效含水量;100风能网)。结果表明,K +高效栽培品种根中GhHAK5aD转录量显著高于K +低效栽培品种。K +缺乏时GhHAK5aD在根中的表达量较高,而在茎中的表达量较低,说明GhHAK5aD可能参与根对K +的吸收,而不是将K +从根转运到茎。大田条件下,在减少灌溉条件下,施钾量使FH-142的籽棉产量(SCY)比BH-212、IUB-2013、CYTO-124和CIM-554分别提高22.3%、4.9%、2.4%和1.4%。在施钾条件下,FH-142的净光合速率比其他减少灌溉的品种提高了57.3%。但在施钾量为50 kg·ha−1的减灌条件下,富氢142、CYTO-124、CIM-554和IUB-2013等钾效型品种的综合性能指标优于钾效型品种BH-212。水分亏缺条件下施钾显著改善了纤维品质性状。在减少灌溉条件下,施用50 kg·ha -1钾肥比不施用钾肥,h -212、IUB-2013、cm -554、CYTO-124和FH-142的麦克隆值分别提高了3.6%、4.7%、7.8%、3.4%和6.7%。与其他品种相比,FH-142在减少灌溉条件下,施钾量增加了12%。减少灌溉条件下,施钾量为50 kg·ha - 1时,高效钾品种的sy和品质性状均比低效钾品种提高30%。富士142的水分利用效率(WUE)为40.1%,钾元素利用效率(KUE)为20.2%,在减量施钾条件下也显著高于其他品种。结论GhHAK5aD基因在棉花根系的表达量在高钾品种中高于低钾品种,表明GhHAK5aD可能是棉花钾离子效率基因型差异的原因之一。低钾环境下可选用高效钾棉花品种,也可作为普通品种的推荐品种。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
7.20
自引率
4.30%
发文量
567
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