Lncrna FGFR3-AS1 Is a Prognostic Indicator for Ovarian Cancer and Induces Cell Proliferation and Hinders Apoptosis

IF 1.4 4区医学 Q3 Medicine

Iranian Journal of Public Health Pub Date : 2023-11-12 DOI:10.18502/ijph.v52i11.14040

Yuyang Zhang, Lili Sun, Tieshuang Chen, Yuanyuan Yue, Lin Zhao, Dan Zhang

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引用次数: 0

Abstract

Background: Ovarian cancer is one of the most common malignant tumors in Gynecology, whose treatment was seriously limited by the unclear understanding of molecular mechanism in disease development. LncRNA FGFR3-AS1 is involved in human cancers. In this study, we aimed to clarify its regulatory effect on ovarian cancer. Methods: Ovarian cell model was used in 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) assay and flow cytometry for investigating the role of LncRNA FGFR3-AS1 in ovarian cancer. Western blot detection (CCND1、CDK6、MAPK9 and PIK3CA) tumor regulatory proteins. The study was conducted between March 2020 and October 2022 at General Hospital of Fushun Mining Bureau of Liaoning Health Industry Group, China. Results: The results demonstrated the upregulation of LncRNA FGFR3-AS1 in ovarian cancer, whose expression was positively related to tumor grade and AJCC stage, and negatively correlated with patients’ prognosis. LncRNA FGFR3-AS1 promoted ovarian cancer development through promoting cell growth and inhibiting cell apoptosis. PI3K signaling pathway-related proteins may be implicated in the LncRNA FGFR3-AS1 induced regulation of ovarian cancer. Conclusion: LncRNA FGFR3-AS1 was involved in the development of ovarian cancer, which has the potential to become therapeutic target and prognostic indicator in ovarian cancer treatment.

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Lncrna FGFR3-AS1是卵巢癌的预后指标，可诱导细胞增殖并阻碍细胞凋亡

背景:卵巢癌是妇科最常见的恶性肿瘤之一，由于对疾病发展的分子机制认识不清，严重限制了卵巢癌的治疗。LncRNA FGFR3-AS1参与人类癌症。在本研究中，我们旨在阐明其对卵巢癌的调控作用。方法:采用3-(4,5 -二甲基噻唑-2-基)- 2,5 -二苯基四唑溴化(MTT)法和流式细胞术建立卵巢细胞模型，研究LncRNA FGFR3-AS1在卵巢癌中的作用。Western blot检测肿瘤调节蛋白(CCND1、CDK6、MAPK9、PIK3CA)。本研究于2020年3月至2022年10月在中国辽宁健康产业集团抚顺矿务局总医院进行。结果:LncRNA FGFR3-AS1在卵巢癌中表达上调，其表达与肿瘤分级、AJCC分期呈正相关，与患者预后负相关。LncRNA FGFR3-AS1通过促进细胞生长和抑制细胞凋亡促进卵巢癌的发展。PI3K信号通路相关蛋白可能参与LncRNA FGFR3-AS1诱导的卵巢癌调控。结论:LncRNA FGFR3-AS1参与卵巢癌的发生发展，具有成为卵巢癌治疗靶点和预后指标的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Public Health 医学-公共卫生、环境卫生与职业卫生

CiteScore

2.20

自引率

7.10%

发文量

审稿时长

2 months

期刊介绍： Iranian Journal of Public Health has been continuously published since 1971, as the only Journal in all health domains, with wide distribution (including WHO in Geneva and Cairo) in two languages (English and Persian). From 2001 issue, the Journal is published only in English language. During the last 41 years more than 2000 scientific research papers, results of health activities, surveys and services, have been published in this Journal. To meet the increasing demand of respected researchers, as of January 2012, the Journal is published monthly. I wish this will assist to promote the level of global knowledge. The main topics that the Journal would welcome are: Bioethics, Disaster and Health, Entomology, Epidemiology, Health and Environment, Health Economics, Health Services, Immunology, Medical Genetics, Mental Health, Microbiology, Nutrition and Food Safety, Occupational Health, Oral Health. We would be very delighted to receive your Original papers, Review Articles, Short communications, Case reports and Scientific Letters to the Editor on the above mentioned research areas.