In vitro inhibition mechanism of Trichoderma asperellum isolates from corn against Rhizoctonia solani causing banded leaf and sheath blight disease and its role in improving the growth of corn seedlings

IF 2.1 3区 农林科学 Q1 ENTOMOLOGY
Hishar Mirsam, None Suriani, Sri Kurniawati, Oky Dwi Purwanto, Amran Muis, Syahrir Pakki, A. Tenrirawe, Nurnina Nonci, None Herawati, None Muslimin, Muhammad Azrai
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引用次数: 1

Abstract

Abstract Background One of the primary corn diseases, banded leaf and sheath blight, is carried on by the pathogenic fungus Rhizoctonia solani . Efforts to control R. solani are more directed at environmentally friendly control using a biological control agent, such as Trichoderma spp. as antagonistic agents and plant growth promoter. This study aimed to identify T. asperellum isolates based on molecular characteristics and to determine the in vitro inhibition mechanism against R. solani and its role in enhancing the growth of corn seedlings. Results The HMRP7, HMRF7A, HMEDF1B, HMEDF6A, and CHM01 isolates were identified as T. asperellum with a genetic distance coefficient value of 0.000 and a very high similarity of 100%. Meanwhile, the RsHM isolate was identified as R. solani with a homology level of > 90% and genetic distance coefficient values ranging from 0.000 to 0.032. The antagonistic ability showed that the five T. asperellum isolates were able to inhibit the growth of R. solani in vitro on PDA medium with an inhibition percentage of ≥ 50%, so they were categorized as antagonist agents. T. asperellum showed the antagonistic mechanism in inhibiting the R. solani growth through the action of parasitism. The five T. asperellum isolates tested on corn seedlings showed a significantly high difference from the control treatment on the observational variables of maximum growth potential (MGP), growth rate (GtR), growth simultaneity (GS), vigor index (VI), germination rate (GR), and median germination time (T50). Conclusion CHM01 isolate showed better potential than other isolates in inhibiting the growth of R. solani in vitro on PDA medium with a parasitism mechanism and enhancing the growth of corn seedlings.
玉米曲霉木霉对带状叶枯病和鞘枯病的体外抑制机制及其对玉米幼苗生长的促进作用
摘要背景玉米带状叶鞘疫病是玉米的主要病害之一,是由枯丝核菌(Rhizoctonia solani)传播的。防治茄蚜的努力更侧重于环境友好型防治,使用生物防治剂,如木霉作为拮抗剂和植物生长促进剂。本研究旨在基于分子特征对asperellum菌株进行鉴定,并探讨其对番茄枯萎病菌的体外抑制机制及其对玉米幼苗生长的促进作用。结果HMRP7、HMRF7A、HMEDF1B、HMEDF6A和CHM01分离株均为曲霉,遗传距离系数为0.000,相似度为100%。与此同时,RsHM分离物经鉴定为茄茄菌(R. solani),同源性水平为>遗传距离系数为0.000 ~ 0.032。拮抗能力表明,5株曲霉菌株在PDA培养基上均能抑制茄枯病菌的生长,抑制率均≥50%,可归类为拮抗剂。曲霉通过寄生作用抑制茄蚜的生长表现出拮抗机制。在玉米幼苗上试验的5株曲霉菌株在最大生长势(MGP)、生长速率(GtR)、生长同时性(GS)、活力指数(VI)、发芽率(GR)和中位发芽时间(T50)等观测变量上均与对照处理存在显著差异。结论与其他菌株相比,CHM01菌株在PDA培养基上具有较好的抑制茄蚜的生长和促进玉米幼苗生长的作用。
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来源期刊
CiteScore
4.90
自引率
16.70%
发文量
128
审稿时长
>12 weeks
期刊介绍: The Egyptian Journal of Biological Pest Control is a periodic scientific journal published by the Egyptian Society for Biological Control of Pests (ESBCP) in collaboration with SpringerNature. The journal aims to publish internationally peer-reviewed, high-quality research articles in the field of biological and integrated pest control (non-chemical control). The journal publishes review articles, original papers, conference reports, book reviews, editorials, laboratory reports, technical notes and short communications.
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