The immunological advantage of owned cats over stray cats: a comparative study of perforin and granzymes gene expressions

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria-Beograd Pub Date : 2023-09-01 DOI:10.2478/acve-2023-0026

Mehmet Cevat Temizkan, Gonca Sonmez, Secil Sevinc Temizkan, Elmas Ulutas, Muhammed Hudai Culha

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引用次数: 0

Abstract

Abstract Some cats live at home with their owners while others live unattended on the streets or in shelters. One might expect that the owned cats would be better prepared for diseases with vaccinations, and good care and feeding conditions whereas the stray cats would be prepared for diseases by constant exposure to microbial factors. However, no study has investigated which group has the stronger immune response against diseases. Cytotoxic T cells and NK cells are known to initiate an immune response that causes apoptosis of the affected cells when stimulated by various factors. This immune response occurs due to an influx of perforin and granzyme proteins into the affected cell. Accordingly, this study compared owned and stray cats in terms of perforin and granzymes gene expression. Blood samples were collected from 30 owned and 30 stray cats, whose health conditions were checked. The samples were analyzed by qPCR for perforin, and granzyme A and granzyme B gene expression. All genes were expressed at a higher level in owned cats, although only the granzyme A gene showed a significant difference (p<0.05). This indicates that this gene plays a more active and significant role in cats than perforin and granzyme B, and that owned cats have a stronger immune response to diseases than stray cats.

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家猫与流浪猫的免疫优势:穿孔素和颗粒酶基因表达的比较研究

一些猫和主人一起生活在家里，而另一些猫则无人看管地生活在街上或收容所。人们可能会认为，养猫的猫会通过接种疫苗、良好的护理和喂养条件为疾病做好更好的准备，而流浪猫则会通过不断接触微生物因素来为疾病做好准备。然而，没有研究调查哪一组对疾病有更强的免疫反应。已知细胞毒性T细胞和NK细胞在受到各种因素刺激时启动免疫反应，导致受影响细胞凋亡。这种免疫反应是由于穿孔蛋白和颗粒酶蛋白流入受影响的细胞而发生的。因此，本研究比较了养猫和流浪猫的穿孔素和颗粒酶基因表达。研究人员采集了30只养猫和30只流浪猫的血液样本，并对它们的健康状况进行了检查。采用qPCR检测穿孔素、颗粒酶A和颗粒酶B基因表达。所有基因在养猫中均有较高的表达水平，只有颗粒酶a基因有显著差异(p < 0.05)。这说明该基因在猫体内的作用比穿孔素和颗粒酶B更为活跃和显著，也说明养猫对疾病的免疫反应比流浪猫更强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Veterinaria-Beograd 农林科学-兽医学

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

18-36 weeks

期刊介绍： The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.