Mehmet Cevat Temizkan, Gonca Sonmez, Secil Sevinc Temizkan, Elmas Ulutas, Muhammed Hudai Culha
{"title":"The immunological advantage of owned cats over stray cats: a comparative study of perforin and granzymes gene expressions","authors":"Mehmet Cevat Temizkan, Gonca Sonmez, Secil Sevinc Temizkan, Elmas Ulutas, Muhammed Hudai Culha","doi":"10.2478/acve-2023-0026","DOIUrl":null,"url":null,"abstract":"Abstract Some cats live at home with their owners while others live unattended on the streets or in shelters. One might expect that the owned cats would be better prepared for diseases with vaccinations, and good care and feeding conditions whereas the stray cats would be prepared for diseases by constant exposure to microbial factors. However, no study has investigated which group has the stronger immune response against diseases. Cytotoxic T cells and NK cells are known to initiate an immune response that causes apoptosis of the affected cells when stimulated by various factors. This immune response occurs due to an influx of perforin and granzyme proteins into the affected cell. Accordingly, this study compared owned and stray cats in terms of perforin and granzymes gene expression. Blood samples were collected from 30 owned and 30 stray cats, whose health conditions were checked. The samples were analyzed by qPCR for perforin, and granzyme A and granzyme B gene expression. All genes were expressed at a higher level in owned cats, although only the granzyme A gene showed a significant difference (p<0.05). This indicates that this gene plays a more active and significant role in cats than perforin and granzyme B, and that owned cats have a stronger immune response to diseases than stray cats.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Veterinaria-Beograd","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/acve-2023-0026","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Abstract Some cats live at home with their owners while others live unattended on the streets or in shelters. One might expect that the owned cats would be better prepared for diseases with vaccinations, and good care and feeding conditions whereas the stray cats would be prepared for diseases by constant exposure to microbial factors. However, no study has investigated which group has the stronger immune response against diseases. Cytotoxic T cells and NK cells are known to initiate an immune response that causes apoptosis of the affected cells when stimulated by various factors. This immune response occurs due to an influx of perforin and granzyme proteins into the affected cell. Accordingly, this study compared owned and stray cats in terms of perforin and granzymes gene expression. Blood samples were collected from 30 owned and 30 stray cats, whose health conditions were checked. The samples were analyzed by qPCR for perforin, and granzyme A and granzyme B gene expression. All genes were expressed at a higher level in owned cats, although only the granzyme A gene showed a significant difference (p<0.05). This indicates that this gene plays a more active and significant role in cats than perforin and granzyme B, and that owned cats have a stronger immune response to diseases than stray cats.
期刊介绍:
The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.