The association of -799C/T and -381A/G matrix metalloproteinase 8 (MMP-8) gene polymorphisms with periodontitis and active MMP-8 levels in the oral cavity

Ioannis Fragkioudakis, Antonis Ntolkeras, Christina Kottaridi, Andreas Grigoriadis, Dimitra Sakellari
{"title":"The association of -799C/T and -381A/G matrix metalloproteinase 8 (MMP-8) gene polymorphisms with periodontitis and active MMP-8 levels in the oral cavity","authors":"Ioannis Fragkioudakis, Antonis Ntolkeras, Christina Kottaridi, Andreas Grigoriadis, Dimitra Sakellari","doi":"10.5937/bjdm2303176f","DOIUrl":null,"url":null,"abstract":"Background/Aim: To investigate the relationship of the -799C/T (rs11225395) and -381A/G (rs1320632) matrix metalloproteinase 8 (MMP8) gene polymorphisms with periodontitis and the levels of active MMP-8 (aMMP-8) in an oral rinse. Material and Methods: Eighty subjects that had participated in a previous study contributed with an oral rinse sample collected before full-mouth periodontal clinical assessments. In addition, aMMP-8 levels in an oral rinse were quantified with a chairside point of care (PoC) test (PerioSafe), and the accompanying digital reader (OraLyzer). After DNA extraction, the samples were amplified with Polymerase Chain Reaction using specific primers. The amplified DNA samples were analyzed with a sequencing assay. Results: All of the patients were homozygous for the -381A/A genotype while 21.2% of the subjects were homozygous for the -799T/T genotype. No significant association was found between the -799T/T and periodontal disease, however, the presence of the specific genotype was significantly associated with the stage of periodontitis and the levels of aMMP-8 in the oral cavity. Conclusions: The results of the current study of 80 Greek subjects indicated that the presence of the rs11225395 (-799 T/T) genotype may be correlated with the severity of the periodontal disease. In addition, patients homozygous for the T/T genotype had higher levels of aMMP-8.","PeriodicalId":488495,"journal":{"name":"Balkan Journal of Dental Medicine","volume":"44 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Balkan Journal of Dental Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5937/bjdm2303176f","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Background/Aim: To investigate the relationship of the -799C/T (rs11225395) and -381A/G (rs1320632) matrix metalloproteinase 8 (MMP8) gene polymorphisms with periodontitis and the levels of active MMP-8 (aMMP-8) in an oral rinse. Material and Methods: Eighty subjects that had participated in a previous study contributed with an oral rinse sample collected before full-mouth periodontal clinical assessments. In addition, aMMP-8 levels in an oral rinse were quantified with a chairside point of care (PoC) test (PerioSafe), and the accompanying digital reader (OraLyzer). After DNA extraction, the samples were amplified with Polymerase Chain Reaction using specific primers. The amplified DNA samples were analyzed with a sequencing assay. Results: All of the patients were homozygous for the -381A/A genotype while 21.2% of the subjects were homozygous for the -799T/T genotype. No significant association was found between the -799T/T and periodontal disease, however, the presence of the specific genotype was significantly associated with the stage of periodontitis and the levels of aMMP-8 in the oral cavity. Conclusions: The results of the current study of 80 Greek subjects indicated that the presence of the rs11225395 (-799 T/T) genotype may be correlated with the severity of the periodontal disease. In addition, patients homozygous for the T/T genotype had higher levels of aMMP-8.
-799C/T和-381A/G基质金属蛋白酶8 (MMP-8)基因多态性与牙周炎和口腔MMP-8活性水平的关系
背景/目的:探讨-799C/T (rs11225395)和-381A/G (rs1320632)基质金属蛋白酶8 (MMP8)基因多态性与牙周炎及口腔冲洗液中活性金属蛋白酶8 (aMMP-8)水平的关系。材料和方法:80名参加过先前研究的受试者在全口牙周临床评估前收集了口腔冲洗样本。此外,通过椅边护理点(PoC)测试(PerioSafe)和随带的数字阅读器(OraLyzer)对口腔冲洗液中的aMMP-8水平进行量化。提取DNA后,用特异性引物进行聚合酶链反应扩增。扩增的DNA样本用测序法进行分析。结果:所有患者-381A/A基因型纯合子,21.2%的患者-799T/T基因型纯合子。没有发现-799T/T与牙周病之间的显著关联,然而,特定基因型的存在与牙周炎的阶段和口腔中aMMP-8的水平显著相关。结论:目前对80名希腊受试者的研究结果表明,rs11225395 (-799 T/T)基因型的存在可能与牙周病的严重程度相关。此外,T/T基因型纯合子的患者aMMP-8水平较高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信