Effect of genotypes on reaction time, refractory period, semen index and liquid stored semen quality of breeding bulls in Bangladesh

Fundamental and Applied Agriculture Pub Date : 2023-01-01 DOI:10.5455/faa.160463

Sharad Chowdhury, Mohammad Mahbubul, Md Habib, Md Ali, Auvijit Apu

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Abstract

The present study was conducted with the aim to reveal the effect of genotypes on reaction time, refractory period, semen index and liquid stored (at 4oC) semen quality of breeding bulls in Bangladesh. Semen was collected twice a week from Holstein Friesian, Sahiwal and Brahman breeding bulls. Reaction time and refractory period were examined for the breeding bulls. Semen volume, sperm concentration and total spermatozoa/ejaculate were measured immediately after collection. It was revealed that genotype had a significant (P<0.01) effect on semen volume, sperm concentration, total sperm output and refractory period but not in reaction time. From the point of view of semen index, semen quality of Holstein Friesian breeding bulls was superior to Sahiwal and Brahnman breeding bulls. Progressive motility, live and normal spermatozoa of fresh semen did not differ significantly but after dilution progressive motility differed significantly (P<0.05) in different genotypes of breeding bulls. During preservation time (0 to 120 hours), progressive motility, normal and live spermatozoa changed significantly (P<0.05) in each genotype with the progress of time. During 0 hour, 72 hours and 120 hours of preservation, progressive motility was found in Holstein Friesian (73.11±1.12%, 53.11±5.14%, 13.40±2.53%), Sahiwal (78.21±1.68%, 64.96±4.60%, 13.22±1.42%), and Brahman (75.21±1.68%, 54.86±4.40%, 16.96±4.42%), respectively. On the other hand, non-return rate was found insignificant (P>0.05), where higher fertility was observed in Holstein Friesian bull (67.2%) followed by Sahiwal (63.7%) and Brahman bull (57.38%). In a nutshell, Holstein Friesian bull has better fresh, diluted and preserved semen quality than the other two genotypes and after the 3 days of preservation, semen quality in respect of progressive motility, normal and live spermatozoa drastically deteriorated. Therefore, it is recommended that preserved semen of different genotypes should be used for AI within 3 days of liquid semen preservation at 4oC.

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基因型对孟加拉国种公牛反应时间、不应期、精液指数和储液精液质量的影响

本研究旨在揭示基因型对孟加拉国种公牛反应时间、不应期、精液指数和液体储存(4℃)精液质量的影响。每周采集两次荷斯坦弗里西亚公牛、萨希瓦尔公牛和婆罗门公牛的精液。测定了种公牛的反应时间和不应期。采集后立即测量精液量、精子浓度和总精子/射精量。结果表明，基因型对精液量、精子浓度、总精子量和不应期有显著影响(P<0.01)，但对反应时间无显著影响。从精液指数来看，荷斯坦弗里西亚种牛的精液质量优于萨希瓦尔种牛和布兰曼种牛。不同基因型种牛新鲜精液的进行性活力、活精子和正常精子差异不显著，但稀释后的进行性活力差异显著(P<0.05)。在保存时间(0 ~ 120 h)内，各基因型精子的进行性运动、正常精子和活精子均随时间的推移发生了显著变化(P<0.05)。保存0小时、72小时和120小时时，Holstein Friesian(73.11±1.12%，53.11±5.14%，13.40±2.53%)、Sahiwal(78.21±1.68%，64.96±4.60%，13.22±1.42%)和Brahman(75.21±1.68%，54.86±4.40%，16.96±4.42%)分别有进行性运动。另一方面，非回报率不显著(P>0.05)，其中荷斯坦弗里斯牛的育仔率较高(67.2%)，其次是萨希瓦尔牛(63.7%)和婆罗门牛(57.38%)。综上所述，荷斯坦阉牛的新鲜、稀释和保存精液质量均优于其他两种基因型，保存3天后，精液质量在进动、正常精子和活精子方面急剧下降。因此，建议在4℃液体精液保存3天内，将不同基因型的保存精液用于人工授精。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fundamental and Applied Agriculture

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审稿时长

12 weeks