Comparative Evaluation of the ID NOW<SUP>TM</SUP> Test (Abott) and RT-PCR for the Detection of the SARS-CoV-2 Genome in Travellers

Ndao Malick, Diagne Babacar, Diagne Rokhaya, Niane Moustapha, Ka Roughyatou
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Abstract

Introduction: The persistence of the COVID-19 pandemic, which has become a global public health problem, means that the implementation of effective and affordable diagnostic strategies is essential, particularly in developing countries, to contain the disease. Rapid, reliable and inexpensive molecular or antigenic tests enable early detection of cases and rapid clinical management. The method based on reverse transcription-polymerase chain reaction (RT-PCR) is the benchmark for diagnosing SARS-CoV-2 infections. However, this method requires highly qualified human resources, complex equipment, consumables and reagents that are usually expensive and imported from developed countries. Given these technical and financial constraints and the limited capacity of molecular platforms in developing countries, point-of-care can be considered a very good alternative. The aim of this study was to evaluate the performance of the ID NOWTM COVID-19 test for the detection of SARS-COV-2 from nasopharyngeal swab samples collected in tubes containing viral transport medium compared with RT-PCR. Method: The evaluation was carried out on 59 travellers from whom a nasopharyngeal swab was taken in 3 ml of viral transport medium (VTM). A swab from the ID-NOW kit was dipped into each sample and then deposited in the sample recipient in order to assess the performance of the ID-NOW test compared with RT-PCR. Results: In our study, we found a sensitivity of 92.6% (23/25) and a specificity of 100%. However, 2 false negatives were found with samples that had CT values of 36. No cross-contamination between samples was observed in this study. Conclusion: Our data showed that the ID NOWTM COVID-19 test would be an excellent tool for screening suspected cases in clinical departments.
ID NOW<SUP>TM</SUP>旅行者SARS-CoV-2基因组检测的abtt法和RT-PCR
导言:COVID-19大流行持续存在,已成为一个全球公共卫生问题,这意味着实施有效和负担得起的诊断战略对于控制这一疾病至关重要,特别是在发展中国家。快速、可靠和廉价的分子或抗原检测有助于早期发现病例和快速临床管理。基于逆转录聚合酶链反应(RT-PCR)的方法是诊断SARS-CoV-2感染的基准。然而,这种方法需要高素质的人力资源、复杂的设备、耗材和试剂,这些通常都很昂贵,而且是从发达国家进口的。考虑到这些技术和财政限制以及发展中国家分子平台的有限能力,医疗点可被视为一个非常好的替代方案。本研究的目的是评估ID NOWTM COVID-19检测方法在含有病毒转运介质的管中采集的鼻咽拭子样本中检测SARS-COV-2的性能,并与RT-PCR进行比较。方法:对59名旅行者进行评估,并在3 ml病毒转运培养基(VTM)中取鼻咽拭子。将ID-NOW试剂盒的拭子浸入每个样品中,然后将其放入样品受体中,以便与RT-PCR相比评估ID-NOW测试的性能。结果:在我们的研究中,我们发现灵敏度为92.6%(23/25),特异性为100%。然而,在CT值为36的样本中发现2例假阴性。本研究未发现样品间的交叉污染。结论:我们的数据显示,ID NOWTM COVID-19检测将是临床科室筛查疑似病例的良好工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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