Check update pattern of tumorigenic vasculature signature based on MMP9 and CXCR4 expression in locally advanced breast cancer

IF 1.2 Q4 PHARMACOLOGY & PHARMACY
Mochamad Bachtiar Budianto, Hamzah Sulaiman Lubis, Muhammad Luqman Fadli, Wiwit Nurwidyaningtyas
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引用次数: 0

Abstract

Context: Locally advanced breast cancers (LABC) are the most common women malignant tumors. Appropriate vasculature is required for tumor growth support, formed by involving protein signaling, including matrix metalloprotein 9 (MMP9) and C-X-C chemokine receptor type 4 (CXCR4). Neoadjuvant chemotherapy (NAC) administration to inoperable LABC commonly exhibits a positive response, although recurrences may be encountered in a few cases. Aims: To evaluate the MMP9 and CXCR4 expression shifting after the NAC procedure to establish evidence of the anti-angiogenic effect of NAC, which encourages knowledge of tumor size reduction pathways in LABC. Methods: Observational designs were conducted in this study. Tissue specimens before and after NAC were collected from 45 LABC-enrolled subjects. The targeted protein expression was analyzed by immunohistochemistry, and stained sections were classified according to the percentage of nuclear-stained tumor cells. Clinicopathological features of LABC were recorded. Tumor size was measured by Vernier caliper before and after NAC. Results: The results showed the nuclear expression of MMP9 and CXCR4 protein were observed in all tissue specimens. The expression of MMP9 and CXCR4 tended to decrease after the NAC but was not statistically significant for MMP9. There was a significant correlation between expression levels of CXCR4 and tumor size reduction (p<0.001) but not for MMP9. Conclusions: The results of this study demonstrate the anti-angiogenic effect of NAC by inhibiting MMP9 and CXCR4, which may be integrated with tumor size reduction in LABC. Further studies are required to highlight the possibility of recurrence following inhibition of MMP9 and CXCR4 by NAC.
检查局部晚期乳腺癌中基于MMP9和CXCR4表达的致瘤性血管特征的更新模式
背景:局部晚期乳腺癌(LABC)是最常见的女性恶性肿瘤。肿瘤生长支持需要适当的血管系统,它是由包括基质金属蛋白9 (MMP9)和C-X-C趋化因子受体4 (CXCR4)在内的蛋白质信号传导形成的。新辅助化疗(NAC)对不能手术的LABC通常表现出积极的反应,尽管在少数情况下可能会复发。目的:评价NAC术后MMP9和CXCR4的表达变化,为NAC抗血管生成作用提供证据,促进对LABC肿瘤缩小途径的认识。方法:本研究采用观察设计。从45名labc入组的受试者中采集NAC前后的组织标本。免疫组化分析靶蛋白表达,并根据核染色肿瘤细胞百分比对染色切片进行分类。记录LABC的临床病理特征。NAC前后用游标卡尺测量肿瘤大小。结果:MMP9和CXCR4蛋白在所有组织标本中均有核表达。NAC后MMP9和CXCR4的表达有降低的趋势,但MMP9的表达无统计学意义。CXCR4的表达水平与肿瘤大小减小之间存在显著相关性(p<0.001),而与MMP9无关。结论:本研究结果表明NAC通过抑制MMP9和CXCR4发挥抗血管生成作用,可能与LABC肿瘤缩小有关。需要进一步的研究来强调NAC抑制MMP9和CXCR4后复发的可能性。
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来源期刊
CiteScore
3.00
自引率
20.00%
发文量
0
审稿时长
8 weeks
期刊介绍: The Journal of Pharmacy & Pharmacognosy Research (JPPRes) is an international, specialized and peer-reviewed open access journal, under the auspices of AVAGAX – Diseño, Publicidad y Servicios Informáticos, which publishes studies in the pharmaceutical and herbal fields concerned with the physical, botanical, chemical, biological, toxicological properties and clinical applications of molecular entities, active pharmaceutical ingredients, devices and delivery systems for drugs, vaccines and biologicals, including their design, manufacture, evaluation and marketing. This journal publishes research papers, reviews, commentaries and letters to the editor as well as special issues and review of pre-and post-graduate thesis from pharmacists or professionals involved in Pharmaceutical Sciences or Pharmacognosy.
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