Partial characterization of digestive proteases in the river prawn Macrobrachium americanum () (Decapoda: Caridea: Palaemonidae) in Mexico

IF 1.2 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Journal of Crustacean Biology Pub Date : 2023-09-01 DOI:10.1093/jcbiol/ruad053

Victor M Laguna-Nataren, Arkady Uscanga-Martínez, Natalia Perales-García, Carlos A Álvarez-González, Francisco J López-Rasgado, José R Díaz-Gallegos, Alexis F Velazco-Ortiz

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Abstract

Abstract We characterized the digestive enzymes in adults of Macrobrachium americanum (Spence Bate, 1868) in southeastern Mexico. The digestive enzyme extracts were made from the specimens’ hepatopancreas. Alkaline proteases, trypsin, chymotrypsin, carboxypeptidase A, leucine aminopeptidase, lipases, α-amylase and alkaline phosphatase activities were determined, as well as the percentage of inhibition, pH, and temperature stabilities through biochemical and electrophoretic techniques. The maximum digestive activity of proteases was at 35 °C and pH 11. Alkaline digestive proteases were highly stable at pHs 4, 9 and 11 at 55 °C after 30 min pre-incubation. PMSF inhibited two bands with proteolytic activity (35.1 and 23.2 kDa), and SBT1 inhibited all bands, including the one with the highest molecular weight (64.2 kDa). We concluded that enzymes in M. americanum coincide with those of omnivorous decapods with a tendency to carnivory.

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墨西哥美洲沼虾(十足目:虾总目:虾科)消化酶的部分鉴定

摘要研究了墨西哥东南部美洲沼虾(Macrobrachium americanum, Spence Bate, 1868)成虫的消化酶。从标本的肝胰腺中提取消化酶提取物。通过生化和电泳技术测定碱性蛋白酶、胰蛋白酶、凝乳胰蛋白酶、羧肽酶A、亮氨酸氨基肽酶、脂肪酶、α-淀粉酶和碱性磷酸酶的活性，以及抑制率、pH和温度稳定性。蛋白酶的消化活性在35℃、pH为11时达到最大值。预孵育30 min后，碱性消化蛋白酶在55°C ph值为4、9和11时高度稳定。PMSF抑制了两条具有蛋白水解活性的条带(35.1和23.2 kDa)， SBT1抑制了所有条带，包括分子量最高的条带(64.2 kDa)。我们得出结论，美洲m.a americanum的酶与杂食性十足动物的酶一致，具有食肉倾向。

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来源期刊

Journal of Crustacean Biology 生物-海洋与淡水生物学

CiteScore

2.10

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Crustacean Biology is the official journal of The Crustacean Society, publishing peer-reviewed research on all aspects of crustacean biology and other marine arthropods. Papers are published in English only, but abstracts or summaries in French, German, Portuguese, or Spanish may be added when appropriate.