Biomimetic construction of oriented lamellar Col/nHAP composite scaffolds and mediation of macrophages to promote angiogenesis and bone regeneration

IF 2.5 4区材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY

Frontiers of Materials Science Pub Date : 2023-11-03 DOI:10.1007/s11706-023-0666-4

Tingting Huang, Tao Huang, Pin Luo, Di Xiao, Yiping Huang, Shenyu Yang, Rong Zeng, Mei Tu

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引用次数: 0

Abstract

Pore characteristics have been identified as key design parameters for osteoimmunomodulation. The strategy reported here is to create an appropriate immune microenvironment by regulating pore characteristics of scaffolds, thereby promoting early angiogenesis and enhancing osteogenesis. A series of collagen/nanohydroxyapatite (Col/nHAP) composite scaffolds with ordered lamellar structures and different layer spacings were prepared by mimicking the ordered lamellar topology of the bone matrix. Our research indicated that the layer spacing and ordered topology of the scaffold exerted an important influence on phenotype transformation of macrophages and the secretion of angiogenic factors. The Col/nHAP-O(135) with large layer spacing not only supported cell attachment and diffusion in vitro, but also promoted early angiogenesis by timely switching from M1 to M2 macrophage phenotype. In vivo data showed that the layer spacing and the ordered structure of the scaffold synergistically regulated the inflammatory response and triggered macrophages to secrete more angiogenesis related cytokines. Col/nHAP-O(135) considerably promoted the neovascularization and new bone formation in the defect site, indicating that Col/nHAP-O(135) could significantly enhance the osteogenic activity of stem cells with the involvement of macrophages.

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定向板层Col/nHAP复合支架的仿生构建及巨噬细胞介导血管生成和骨再生

孔隙特征已被确定为骨免疫调节的关键设计参数。本文报道的策略是通过调节支架的孔隙特性来创造适当的免疫微环境，从而促进早期血管生成和增强骨生成。通过模拟骨基质的有序板层结构，制备了一系列具有有序板层结构和不同层间距的胶原/纳米羟基磷灰石(Col/nHAP)复合支架。我们的研究表明，支架的层间距和有序拓扑结构对巨噬细胞的表型转化和血管生成因子的分泌有重要影响。大层间距的Col/nHAP-O(135)不仅支持细胞在体外的附着和扩散，而且通过巨噬细胞表型及时从M1向M2转换，促进早期血管生成。体内数据显示，支架的层间距和有序结构协同调节炎症反应，并触发巨噬细胞分泌更多与血管生成相关的细胞因子。Col/nHAP-O(135)可显著促进缺损部位的新生血管和新骨形成，说明Col/nHAP-O(135)可显著增强巨噬细胞参与的干细胞的成骨活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers of Materials Science MATERIALS SCIENCE, MULTIDISCIPLINARY-

CiteScore

4.20

自引率

3.70%

发文量

515

期刊介绍： Frontiers of Materials Science is a peer-reviewed international journal that publishes high quality reviews/mini-reviews, full-length research papers, and short Communications recording the latest pioneering studies on all aspects of materials science. It aims at providing a forum to promote communication and exchange between scientists in the worldwide materials science community. The subjects are seen from international and interdisciplinary perspectives covering areas including (but not limited to): Biomaterials including biomimetics and biomineralization; Nano materials; Polymers and composites; New metallic materials; Advanced ceramics; Materials modeling and computation; Frontier materials synthesis and characterization; Novel methods for materials manufacturing; Materials performance; Materials applications in energy, information and biotechnology.