DIVERSITY AND ABUNDANCE OF CULTURABLE VIBRIONACEAE IN THE REARING TANKS OF THREE DIFFERENT CONSUMER TYPES OF AQUACULTURE COMMODITIES

H. Munandar, Dien Arista Anggorowati, Varian Fahmi, Syafriyadi Hafid
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Abstract

Vibrio spp. bacteria species are heterotrophic and halophilic bacteria, which are commonly found in various habitats such as brackish, marine waters and animals. They are widely used as an indicator of water quality and cultured animal health. This study investigates the diversity and abundance of Vibrionaceae populations in the rearing water of three distinct consumer types of aquaculture commodities, namely abalone, lobster and sea cucumber, during six months sampling. We isolated Vibrionaceae bacteria from the rearing tanks of three different consumer types of aquaculture commodities. We then inoculated 100 µL of undiluted to 10-5 diluted water samples onto petriplate containing selective Thiosulphate Bile Salt Sucrose agar (TCBS). We characterised bacteria colonies based on the biochemical test for identification. Vibrio metschnikovii was the predominant bacteria species in abundance value from the rearing water of cultured lobster and sea cucumber. Meanwhile, Vibrio anguillarum was the most noticeable bacteria species in abundance from the rearing water of cultured abalone. These data provide information and facilitate the risk assessment of these pathogenic Vibrio species in aquaculture commodities.
三种不同消费类型水产养殖商品饲养池中可培养弧菌科的多样性和丰度
弧菌属细菌属异养性和嗜盐性细菌,常见于各种生境,如咸淡水、海水和动物。它们被广泛用作水质和养殖动物健康的指标。本研究调查了鲍鱼、龙虾和海参这三种不同消费类型水产养殖商品的养殖水中弧菌科种群的多样性和丰度。我们从三种不同消费类型水产养殖商品的饲养池中分离出弧菌科细菌。然后将100µL未稀释至10-5稀释的水样接种到含有选择性硫代硫酸盐胆汁盐蔗糖琼脂(TCBS)的培养皿上。我们在生化试验的基础上对菌落进行了鉴定。在养殖龙虾和海参水体中,丰度值优势菌种为梅氏弧菌。同时,鳗鲡弧菌是养殖鲍鱼水体中丰度最高的菌种。这些数据提供了信息并促进了对水产养殖商品中这些致病性弧菌物种的风险评估。
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