Enzymatic Glucose Biosensor of Buckypaper; an engineered surface of CNT

Abstract

Much recent researches have focused on electrochemical biosensor s to meet the growing demands for rapid and accurate diagnosis tests for disease. Conventional enzyme-based sensors propose favorably selective and sensitive determination of glucose at the outlay of low stability. Thus, promoting the comfortable, sensitive, rapid and consistent strategies has an impressive role for determining the human glucose level. Here, we synthesis a novel macro scale network of carbon nanotubes “Buckypaper” by a non-ionic surfactant (Tween 20). Buckypaper (BP) is a plate-like carbon nanotube (CNT) films obtained from single-walled CNTs (SWCNTs) in a form of entangled networks of ropes and bundles of CNT held together by van der Waals. The BP was characterized by Fourier transform infrared spectroscopy (FTIR) and Field emission scanning electron microscopy (FESEM). The electrochemical behavior and electrical conductivity of the BP was investigated through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).BP shows a significant electrochemical result compared with the Carbon nanotube and Graphene. The BP was utilized as a surface for glucose detection with a linear range of 0.1×10−3 – 13×10−3M and the electrochemical response of the interfering species was also examined and well-defined glucose response was obtained and insignificant response were observed for interfering species. The sensor exhibited excellent performance for glucose at applied potential of 0.575V. To the best of our knowledge, on the first researches on the Buckypaper for biosesning application as an individual surface for enzyme stabilization and enzymatic sensing without functionalization .