Characterization of amylase, lipase and xylanase produced by actinobacteria cultivated in licuri [Syagrus coronata - (Martius) Beccari] residues

Abstract

In order to seach for different microbial sources of enzymes, Arthrobacter polychromogenes CDPI-30 and Streptomyces violaceoruber CDPA-32 were individually cultivated in licuri [Syagrus coronata - (Martius) Beccari] residues. It was possible to detect some enzymes but amylase, lipase and xylanase were selected for a basic characterization. The optimum conditions were observed at pH 6 and 7 and 40 – 70 °C for amylases, pH 4 – 6 and 50 – 90 °C for lipases and pH 8 and 10 and 20 – 50 °C for xylanases. The best stabilities, evaluated under different conditions were, in a way, a result from the optimal conditions. Additionally, substrate saturation was not observed for soluble starch and xylan until 15 mg/mL and p-nitrophenyl palmitate until 9.95 µmol/mL. These results indicate that both, actinobacteria and the agroindustrial residues, are good choices to produce basic industrial enzymes.