REPELATION POTENCY OF N-HEXANE EXTRACT OF BASIL LEAF AND STEM (OCIMUM BASILICUM L.) AGAINST CULEX QUINQUEFASCIATUS

I. Ramayanti, Miranti Dwi Hartanti, Reynaldi Aulia Rahman
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Abstract

The Culex quinquefasciatus mosquito is the main vector of filariasis and a popular effort to control it in society is by using chemical materials, but they cause toxic effects in humans, so it is necessary to use a repelant from natural ingredients, one of which is basil containing essential oils and proven to be effective as a repelant against mosquito. The objective of this research was to determine repelant power of n-hexane extract of basil leaves and stems against the Culex quinquefasciatus. This study was a laboratory experimental study using a completely randomized design, with 3 replications at 7 time intervals. The extract concentrations were 15%, 25%, 35 % and ethanol negative control 96%, the positive control repelant X containing 13% DEET. The results showed that the n-hexane extract of basil leaves and stems for 6 hours at a concentration of 15% was able to resist 75% of mosquito, a concentration of 25% was able to resist as much as 85.05%, and a concentration of 35% was able to resist as much as 92.51%,  the one-way ANOVA analysis showed that the value of p = 0.000 (p<0,05), meaning that there was a difference in the number of Culex quinquefasciatus perching on the black cloth at various concentrations of n-hexane extracts of leaves and stems of basil, while the probit analysis showed that the effective extract concentration at 90% was 31.52%. The extract of n-hexane leaves and basil stems was effective as a repelant against the Culex quinquefasciatus.
罗勒叶和茎正己烷提取物的驱虫力研究对抗致倦库蚊
致倦库蚊是丝虫病的主要传播媒介,社会上常用的防治方法是使用化学物质,但化学物质对人体有毒性作用,因此有必要使用天然成分的驱蚊剂,其中一种是含有精油的罗勒,已被证明是有效的驱蚊剂。本研究的目的是确定罗勒叶和茎正己烷提取物对致倦库蚊的驱避力。本研究为实验室实验研究,采用完全随机设计,在7个时间间隔内进行3次重复。提取液浓度分别为15%、25%、35%,乙醇为阴性对照96%,阳性对照驱蚊剂X含避蚊胺13%。结果表明,罗勒叶茎正己烷提取液在浓度为15%的条件下处理6 h,对蚊虫的抑制率为75%,25%浓度的抑制率高达85.05%,35%浓度的抑制率高达92.51%,单因素方差分析显示p = 0.000 (p< 0.05);说明不同浓度罗勒叶、茎正己烷提取物对致倦库蚊在黑布上的栖息数量存在差异,概率分析表明,90%时的有效提取物浓度为31.52%。正己烷叶和罗勒茎提取物对致倦库蚊有较好的驱避效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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