Surface-Enhanced Raman Spectroscopy and Transfer Learning Toward Accurate Reconstruction of the Surgical Zone

Ashutosh Raman, Ren A. Odion, Ken Yamamoto, Weston A. Ross, P. Codd, T. Vo‐Dinh
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Abstract

Raman spectroscopy is a photonic modality defined as the inelastic backscattering of excitation coherent laser light. It is particularly beneficial for rapid tissue diagnosis in sensitive intraoperative environments like those involving the brain, due to its nonionizing potential, point-scanning capability, and highly-specific spectral fingerprint signatures that can characterize tissue pathology [1]. While Raman scattering is an inherently weak process, Surface-Enhanced Raman Spectroscopy (SERS), which is based on the use of metal nanostructure surfaces to amplify Raman signals, has become a compelling method for achieving highly specific Raman spectra with detection sensitivity comparable to conventional modalities such as fluorescence [2]. A unique plasmonics-active nanoplatform, SERS gold nanostars (GNS) have previously been designed in our group to accumulate preferentially in brain tumors [2]. Raman detection, when combined with machine learning and robotics, stands to enhance the diagnosis of ambiguous tissue during tumor resection surgery, with the potential to improve extent-of-resection and rapidly reconstruct the dynamic surgical field. Here we demonstrate preliminary results from the use of a SERS-based robotics platform to efficiently recreate a tumor embedded in healthy tissue, which is modeled here as a GNS-infused phantom. Transfer learning, specifically through use of the open-source RRUFF mineral database, is employed here to address the dearth of collected biomedical Raman data [3].
表面增强拉曼光谱和迁移学习对手术区域的精确重建
拉曼光谱是一种光子模态,定义为激发相干激光的非弹性后向散射。由于其非电离电位、点扫描能力和可表征组织病理特征的高度特异性光谱指纹特征,它特别有利于在敏感的术中环境(如涉及大脑的环境)中快速诊断组织。虽然拉曼散射是一个固有的弱过程,但基于使用金属纳米结构表面来放大拉曼信号的表面增强拉曼光谱(SERS)已经成为一种引人注目的方法,用于实现高度特异的拉曼光谱,其检测灵敏度可与荧光[2]等传统模式相媲美。一种独特的等离子体活性纳米平台,SERS金纳米星(GNS)在我们的团队之前设计过,可以优先在脑肿瘤[2]中积累。当拉曼检测与机器学习和机器人技术相结合时,可以增强肿瘤切除手术中模糊组织的诊断,有可能提高切除范围并快速重建动态手术场。在这里,我们展示了使用基于sers的机器人平台来有效地重建嵌入健康组织中的肿瘤的初步结果,该肿瘤在这里被建模为gns注入的幻影。迁移学习,特别是通过使用开源的RRUFF矿物数据库,在这里被用来解决收集的生物医学拉曼数据的缺乏问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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