Classification of breast-tissue microarray spots using colour and local invariants

Abstract

Breast tissue microarrays facilitate the survey of very large numbers of tumours but their scoring by pathologists is time consuming, typically highly quantised and not without error. Automated segmentation of cells and intra-cellular compartments in such data can be problematic for reasons that include cell overlapping, complex tissue structure, debris, and variable appearance. This paper proposes a computationally efficient approach that uses colour and differential invariants to assign class posterior probabilities to pixels and then performs probabilistic classification of TMA spots using features analogous to the Quickscore system currently used by pathologists. It does not rely on accurate segmentation of individual cells. Classification performance at both pixel and spot levels was assessed using 110 spots from the adjuvant breast cancer (ABC) chemotherapy trial. The use of differential invariants in addition to colour yielded a small improvement in accuracy. Some reasons for classification results in disagreement with pathologist-provided labels are discussed and include noise in the class labels.