Species Checklistand DNA Barcoding of Baung (Bagrid Catfish) Hemibagrus Hoevenii from Muar River, Johor

Abstract

Of Asia, Africa, and the Middle East, there are 15 genera in the Bagridae family. The tropical freshwater catfish Hemibagrus hoeveniiis found in Asian waters. Bagrids are also known as Old World pimelodids, while New World bagrids may be more accurate. In Muar, Johor, DNA barcoding has never been utilised to determine the species of bagrid catfish. Therefore,this study was done to updatethe species checklist of Baung (bagrid catfish)in Muar River,and DNA barcoding of protein-coding cytochrome coxidase I (COI) mitochondrial gene was donefor species identification and phylogenetic analyses. A number of two partial COI gene sequencesranging 674-687 nucleotide baseswere successfully obtained for two specimens of Baung Lawi and the Nucleotide Basic Local Alignment Search Tool (BLAST) analysis suggested theirspecies status as from the genus Hemibagrus. Furthermore, the results of the phylogenetic analyses showedthat the neighbour joining tree, the maximum parsimony tree and the maximum likelihood tree grouped the COI mtDNA gene sequencesof Baung Lawifrom MuarRiverin one single cluster, thusconfirming the species status and showed the presence of H. hoeveniiin MuarRiver, Johor.