Anti-Inflammatory and Antioxidant Activity of an Extract of Luzula sylvatica in a Co-Culture Model of Fibroblasts and Macrophages

Abstract

Background: Plants are a promising source of innovative bioactive compounds for skin treatment and some species of the Juncaceae family have been used in folk medicine for the treatment of erysipelas or bleeding. We focused on Luzula sylvatica, a Juncaceae member that has sparked growing interest for its anti-inflammatory potential Methods: The antioxidant properties of an ethanolic extract of Luzula sylvatica (LS-E) were tested with a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and Oxygen Radical Absorbance Capacity (ORAC) assay. The LS-E was then tested on human dermal fibroblast (HDFa) migration, THP-1 macrophage polarization, and a co-culture model of macrophages and fibroblasts Results: The LS-E showed strong DDPH and ORAC values (1,057 μM and 4,954 μM Trolox equivalent, respectively). The LS-E (50 μg/mL) significantly inhibited IL1β expression in pro-inflammatory polarized macrophages and decreased their secretion of PGE2. The co-culture of fibroblasts with inflammatory M1 macrophages increased proteases gene expression of the fibroblasts. The addition of LS-E dampened the expression of the MMP9 significantly whereas it increased COL1A2 by 2.3 Conclusions: Luzula sylvatica exhibited good antioxidant properties and altered the pro-inflammatory polarization of macrophages. By adding the inhibition of expression of proteases in fibroblasts, these results suggest that Luzula sylvatica shows interesting skin protective activity. Citation: Cholet J, Decombat C, Vareille-Delarbre M, Gainche M, Berry A (2022) Anti-Inflammatory and Antioxidant Activity of an Extract of Luzula sylvatica in a Co-Culture Model of Fibroblasts and Macrophages. Curr Res Cmpl Alt Med 6: 152. DOI: 10.29011/2577-2201.100052 2 Volume 06; Issue 01 Curr Res Complement Altern Med, an open access journal ISSN: 2577-2201