Isolation and Characterization of Resistant Bacterial Species Isolated From Shallow Well Water Situated Close to Graves as a Public Health Menace in Osogbo, Osun State

Stamford Journal of Microbiology Pub Date : 2022-12-20 DOI:10.3329/sjm.v12i1.63344

P. Ozabor, OO Oluwajide, AO Akeju, SJ Onifade, S. Olaniyan, J. Olaitan

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Abstract

This study was designed to evaluate the physicochemical and bacteriological qualities of well water situated close to graves. Total heterotrophic bacterial count (THBC), total coliform count (TCC) and fecal coliform count (FC) were done on nutrient agar, MacConkey and Eosine methylene blue agar, respectively, using spread plate method at incubation conditions of 37℃ for 24 hours for THBC and TCC whereas 44.5℃ for 24-48 hours for fecal coliforms. Membrane filtration was used for Vibrio spp. count on TCBS agar while conventional biochemical method was performed for bacterial identification. Isolated bacteria were subjected for antimicrobial resistant testing using 8 families of antibiotics. Bacteria with MAR index value of ≥75 were sent for genomic identification and sequencing. The average value of the ten water samples analyzed over the period of study had high total suspended solids (mg/l), phosphate (mg/l); magnesium ion (mg/l) contents and a very low dissolved oxygen mg/L content. The THB count was (5.0×107-1.35×108 CFU/ml), TCC count (3.55×105- 1.04×106 CFU/ml), FC count (2.10×105-6.90×105 CFU/ml) while Vibrio spp. count was 45-144 CFU/100 ml and the MPN estimated reading for coliform was also high. The percentage occurrence and MAR index value of the bacteria isolated from the well water, respectively were Vibrio spp. 23%; 75, Klebsiella spp. 20%; 100, Bacillus spp. 14%; 100, Staphylococcus spp. 14%; 62.5, E. coli 12%; 62.5, Pseudomonas spp. 12%; 75, Glycomyces spp. 3%; 100 and Proteus spp. 3%; 50. The closeness of the well to the grave makes the decomposing leachates with high organic, inorganic, biological and poisonous metals sink into the underground aquifers. Stamford Journal of Microbiology, Vol.12 (1) 2022: 47-53

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奥孙州Osogbo市靠近坟墓的浅井水中作为公共卫生威胁的耐药细菌的分离和特性

本研究旨在评估靠近坟墓的井水的理化和细菌学品质。采用铺板法分别在营养琼脂、MacConkey琼脂和Eosine亚甲基蓝琼脂上测定总异养细菌计数(THBC)、总大肠菌群计数(TCC)和粪便大肠菌群计数(FC)，培养条件为37℃孵育24小时，粪便大肠菌群孵育条件为44.5℃孵育24-48小时。采用膜过滤法对TCBS琼脂上弧菌进行计数，采用常规生化法对细菌进行鉴定。采用8科抗生素对分离细菌进行耐药试验。对MAR指数≥75的细菌进行基因组鉴定和测序。在研究期间分析的10个水样的平均值具有高的总悬浮固体(mg/l)，磷酸盐(mg/l);镁离子(mg/l)含量和溶解氧(mg/l)含量极低。THB计数为(5.0×107-1.35×108 CFU/ml)， TCC计数为(3.55×105- 1.04×106 CFU/ml)， FC计数为(2.10×105-6.90×105 CFU/ml)，弧菌计数为45-144 CFU/100 ml，大肠菌群的MPN估计读数也很高。井水分离细菌的出现率和MAR指数值分别为弧菌(Vibrio spp.)的23%;克雷伯氏菌75例，20%;100，芽孢杆菌，14%;100，葡萄球菌，14%;62.5，大肠杆菌12%;62.5，假单胞菌属12%;75、糖酵母菌属3%;100和变形杆菌属3%;50. 由于井与坟墓的距离较近，使得分解的渗滤液中含有大量有机、无机、生物和有毒金属，渗入地下含水层。微生物学报，Vol.12 (1)， 2022: 47-53

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Stamford Journal of Microbiology

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