Difference proportional cell contact platform for 3D hepatocyte culture

2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2012-03-05 DOI:10.1109/NEMS.2012.6196754

Yu-Shih Chen, Zong-Keng Kuo, L. Ke, Chiou-Wen Lin, Hsiang-Wen Tseng, Cheng-Hsien Liu

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Abstract

This paper reports a difference proportional cell contact platform for three dimension hepatocyte culture. We designed the cell wires about the width of 25 micrometer by dielectrophoresis for difference contact area. The platform was consisted of two dimensional cell contact chambers and three dimensional liver tissue chambers by dielectrophoresis pattern method and microfluidic channel design. For cell contact chambers, we used dielectrophoresis pattern method to make difference contact area for difference proportional cell communication. For liver tissue chambers, we used the UV-initiated solution of the poly (ethylene glycol)-diacrylate (PEG-DA) had been exposed to form microwells to pattern three dimension lobule-mimetic structure liver tissue in vitro. HepG2 cells were loading with three dimensional PEG-DA-dased micro-structures for artificial lobule pattern. Finally, we showed liver functional albumin testing. The albumin secretion of liver pattern chambers with HMEC-1 and C2C12 co-culture communication was 34% higher than the albumin secretion of liver chip without cells communication.

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三维肝细胞培养的差分比例细胞接触平台

本文报道了一种用于肝细胞三维培养的差分比例细胞接触平台。采用电泳法设计了25微米宽的胞丝，以达到不同的接触面积。采用介质电泳法和微流控通道设计，构建了二维细胞接触室和三维肝组织室。对于细胞接触室，我们采用介电图法制造不同的接触面积，以实现不同比例的细胞通信。对于肝组织腔室，我们使用uv引发的聚乙二醇-二丙烯酸酯(PEG-DA)溶液暴露形成微孔，在体外模拟三维小叶结构的肝组织。在HepG2细胞上加载三维peg -da微结构，形成人造小叶图案。最后，我们进行了肝功能白蛋白检测。HMEC-1和C2C12共培养通讯的肝模式室的白蛋白分泌量比无细胞通讯的肝芯片的白蛋白分泌量高34%。

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来源期刊

2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

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