An impedance probing system for real-time intraoperative brain tumour tissue discrimination

Abstract

The ability to acquire realtime diagnostics of brain tissue intraoperatively represents a key goal in the field of brain tumour neurosurgery. This can greatly enhance the precision, extent and effectiveness of key surgical procedures such as those performed for brain tumour resection and biopsy. To achieve this requires a miniature, handheld tool which can perform intraoperative in situ, in-vivo characterisation of different types of tissues e.g. normal brain tissue versus tumour tissue. Here we explored the feasibility and requirements of implementing a portable impedance characterisation system for brain tumour detection. We proposed and implemented a novel system based on PCB-based instrumentation using a square four-electrode microendoscopic probe. The system uses a digital-to-analogue converter to generate a multi-tone sinusoid waveform, and a floating bi-directional voltage-to-current converter to output the differential stimulation current to one pair of electrodes. The other pair of electrodes are connected to the sensing circuit based on an instrumentation amplifier. The recorded data is pre-processed by the micro-controller and then analysed on a host computer. To evaluate the system, tetrapolar impedances have been recorded from a number of different electrode configurations to sense pre-defined resistance values. The overall system consumed 143mA current, achieved 0.1% linearity and 15µV noise level, with a maximum signal bandwidth of 100kHz. Initial experimental results on tissue were carried out on a piece of rib-eye steak. Electrical impedance maps (EIM) and contour plots were then reconstructed to represent the impedance value in different tissue region.