Functionality and Clinical Effects of Anti-Cov2 Vaccines (Aka Mrna) And Integration on Mitochondrial DNA

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Abstract

1. This issue would require many pages, but it is briefly described here for easier understanding 2. The writer refers to the Molecular Biology Experts and to reading the copious literature on the proteins/ enzymes found in mRNA vaccines using the BLASTP approach (Appendix 1) 3. The reference literature, apart from being written in English, is also worded in an extremely technical style 4. They are given a specific functional sequence, aimed at the incorporation and expression of vaccine mRNA into the genome of the mitochondria (mtDNA) 5. The vaccine mRNA does not refer to the segment relating to the Spike protein, but to the entire viral genome 6. Therefore, the Ribosomes are not the final destination of the vaccine mRNA; instead, the Mitochondria are actually the seat of a DNA, accommodating the SARS-Cov2 genome in the same way as it does in the nuclear genome. 7. Both the Mitochondria and Ribosomes are located in the Ergastoplasm and are separated from the cytosol by an oxidation/reduction barrier 'in mV' (= one thousand times) three times higher than that of the cytosol. 8. Article from Science: Oxidized Redox State of Glutothiane in the Endoplasmic reticulum - 11 September 1992 volume 257, page 1496:15026 9. Thus only an mRNA that exits from both the nuclear and mitochondrial genomes has the enzyme support that allows it to reach the ribosomes, overcoming the resistance of the oxidation/reduction barrier 10. If this were not the case, RNA viruses would not lengthen the pathway, integrating before the genome and then reaching the ribosomes again as mRNA (it must be said that, in an RNA virus, the genomic RNA is the same as the RNA coming out of the nucleus), but would immediately aim to reach the ribosomes, as RNA vaccines are said to do. 11. Nano technologies are said to be the architects of this miraculous and rapid rush to the ribosomes of vaccine mRNA 12. These nano-technologies are nothing more than a sequence of proteins/enzymes - undeclared - which are now described here in their function in the main patterns 13. a) ryanodine receptorby managing the Ca++ ion flux channels, it first depolarises the mitochondrial membrane, which is the first step for mRNA entry and then it lowers the oxidative/reductive level of the ergastoplasm, activating an osmotic "Δ" (= delta-difference) around the mitochondria, releasing the protonated ions (=H + ) present in the virtual space of the two sheets forming the mitochondrial membrane into the area. 14. b) ABC transporter - ATP - binding protein By binding to ATP, it allows vaccine RNA to enter the mitochondria 15. c) Epical complex lysine methyltran spherase By providing mobility for the entering mRNA, it allows to reach the stretch of mitochondrial DNA most suitable for integration 16. d) DDLS - Typ - integrase/transpasase It simultaneously does the work performed before by reverse transcriptase and integrase, and then inserts a viral genome RNA, similar to the genomic RNA in RNA viruses, into the mitochondrial genome. 17. e) Pullulanase The hydrolysis of the (1 → 6) X - D glucoside bonds of mitochondrial DNA paves the way for the action of the
抗cov2疫苗(又名Mrna)的功能和临床效果及其对线粒体DNA的整合
1. 这个问题需要很多页的篇幅,但为了更容易理解,这里简单地描述一下。作者参考了分子生物学专家,并阅读了大量关于使用BLASTP方法在mRNA疫苗中发现的蛋白质/酶的文献(附录1)3。参考文献,除了用英语写的,也是用一种非常专业的风格。它们被赋予特定的功能序列,目的是将疫苗mRNA整合并表达到线粒体(mtDNA)基因组中5。疫苗mRNA不是指与Spike蛋白相关的片段,而是指整个病毒基因组6。因此,核糖体不是疫苗mRNA的最终目的地;相反,线粒体实际上是DNA的所在地,以与核基因组相同的方式容纳SARS-Cov2基因组。7. 线粒体和核糖体都位于细胞质中,并通过比细胞质高3倍的氧化/还原屏障(mV = 1000倍)与细胞质分离。8. 文章来自科学:内质网中谷胱甘肽的氧化还原状态- 1992年9月11日卷257,页1496:15026因此,只有从核和线粒体基因组中同时存在的mRNA才有酶的支持,使其能够到达核糖体,克服氧化/还原屏障的阻力。如果不是这样,RNA病毒就不会延长途径,在基因组之前整合,然后以mRNA的形式再次到达核糖体(必须说,在RNA病毒中,基因组RNA与从细胞核中出来的RNA是一样的),而是会立即以到达核糖体为目标,就像RNA疫苗所说的那样。11. 据说,纳米技术是疫苗mRNA 12核糖体奇迹般快速进入核糖体的建筑师。这些纳米技术只不过是蛋白质/酶的序列——未声明——现在在这里以主要模式描述它们的功能。a) ryanodine受体通过管理Ca++离子通量通道,首先使线粒体膜去极化,这是mRNA进入的第一步,然后降低线粒体质的氧化/还原水平,激活线粒体周围的渗透性“Δ”(= Δ差),将存在于形成线粒体膜的两片虚拟空间中的质子化离子(=H +)释放到该区域。14. b) ABC转运蛋白- ATP结合蛋白通过与ATP结合,它允许疫苗RNA进入线粒体。通过为进入的mRNA提供移动性,它可以到达最适合整合的线粒体DNA片段16。d) DDLS - type -整合酶/转酶它同时完成之前通过逆转录酶和整合酶完成的工作,然后将病毒基因组RNA插入线粒体基因组,类似于RNA病毒中的基因组RNA。17. e)普鲁兰酶线粒体DNA(1→6)X - D糖苷键的水解为酶的作用铺平了道路
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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