Correlation between Soil Phospor (P) Content and Soil Yeast Antagonistics Ability Against Fusarium oxysporum Pathogen Causing Wilt

A. Muhibuddin, Kartika Novitasari Wibowo, H. Punnapayak, P. Goetz
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Abstract

Besides its role during fermentation process, yeast also has potential as biological agent because of its antagonistics characteristic. We have isolated yeast from six different locations based on Phospourus differences. Isolated yeast were then tested its antagonistics ability of Fusarium oxysporum, the pathogen causing tomato wilt disease. This research aims to find out the diversity of yeast found in the tomato rhizosphere in six different contents of phosphorus locations and to know its antagonistics ability against the pathogenic. F. oxysporum. This research was conducted in the Laboratory of Mycology, Department of Pests and Diseases, Faculty of Agriculture, Brawijaya University, Malang and in the Chemistry Laboratory, Institut Teknologi 10 Nopember Surabaya, started from January up to September 2015. Yeasts have been isolated from tomato’s rhizosphere of 6 different locations around East Java proviences. The result showed that yeasts from organic field (Lower P content) were 6 genera. They are Candida sp. 1, Pichia sp. 1, Hansenula sp., Metschnikowia sp. 1, Cryptococcus sp., and Zygosaccharomyces sp. While the yeasts from inorganic field (higher P) were 3 genera. The most potential yeast in controlling F. oxysporum is Pichia sp. 2. Lower P content showed more divers than higher P content. Yeasts from higher soil P content showed more antagonists to control F. oxysporum. Keywords: Phosphor, correlation, yeast, antagonistic, disease, soil
土壤磷含量与土壤酵母菌对枯萎病病原菌拮抗能力的关系
酵母除了在发酵过程中发挥作用外,还因其拮抗特性而具有作为生物制剂的潜力。根据磷的不同,我们从六个不同的地点分离出酵母。分离酵母对番茄枯萎病病原菌尖孢镰刀菌的拮抗能力进行了试验。本研究旨在了解番茄根际酵母在6个不同磷含量位置的多样性,并了解其对病原菌的拮抗能力。病圃。该研究于2015年1月至9月在玛琅Brawijaya大学农学院病虫害系真菌学实验室和泗水理工学院化学实验室进行。从东爪哇省6个不同地点的番茄根际分离出酵母。结果表明,有机田产酵母(磷含量较低)有6属。其中念珠菌1属、毕赤酵母1属、汉氏菌1属、Metschnikowia 1属、隐球菌1属、Zygosaccharomyces sp.,无机场(高P)酵母3属。在控制尖孢镰刀菌方面最有潜力的酵母是毕赤酵母2。低磷含量比高磷含量表现出更多的潜水员。土壤磷含量高的酵母对尖孢镰刀菌的拮抗作用更强。关键词:磷,相关,酵母,拮抗,疾病,土壤
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