Examining the Effect of Extract of Oak Fruit Jaft on AGS Cell Lines

Abstract

Background: Gastrointestinal carcinoma comprises 5% of all pediatric cancer in children. Given that the possible and beneficial effect of the Jaft extract in the treatment of gastric cancer is not known and there is no comprehensive study in this regard, this study aimed to assess the effect of Jaft extract on gastric cancer cell lines. Materials and Methods: In this case-control study, oak fruit was collected from the mountains of Lorestan province. A gastric cancer (AGS) cell line was obtained from the Institute Pasteur cell bank and was cultured. After the preparation of the ethanolic extract of Jaft, the cell viability of the gastric cancer cells treated with Jaft extract was investigated by MTT assay. Quantitative Reverse Transcription PCR was used for assessing the expression of BAX, and BCL2 genes. Results: The half maximal inhibitory concentration (IC50) value of Jaft extract was 162 µg/ml. The BAX gene expression was different between the case and control groups. In this regard, the expression of the BAX gene was increased in the concentration of 162 (P<0.01) and 250 µg/ml (P<0.001) of Jaft extract compared to the control group. The BCL2 expression was different between the two groups (P<0.05). In this regard, the expression of the BCL2 gene was decreased in the concentration of 162 and 250 µg/ml of Jaft extract compared to the control group. Conclusion: It was found that Jaft extract increased the apoptosis of gastric cancer cells; therefore, it seems that the hydroalchoholic extract of Jaft is an appropriate anticancer medication.