THE INFLUENCE OF THE REACTION MEDIUM ON THE EXTRACTION PROCESS OF GLYCOLIPIDS OF THE PSEUDOMONAS BACTERIA

Abstract

Optimization of the technology of isolation of biogenic surface-active products of microbial synthesis is an actual problem of modern chemistry and biotechnology. In this work, the processes of extraction of surface-active glycolipids from the cultural liquid supernatant of bacterial strains Pseudomonas aureofaciens NB-1 and Pseudomonas fluorescens 8573 were investigated. The most problematic stage in the production of biosurfactants is their isolation from the culture liquid. Even with optimized biosynthesis, the production efficiency depends heavily on rational methods of isolation of the target products. In this connection, there is a growing need for the optimization of the processes of isolation of biosurfactants. It main isolate biosurfactants from the supernatant to transfer them the of an organic solvent. However, biosurfactants have a complex structure; therefore, their extraction is important and difficult established, extraction of glycolipids from the cultural of Pseudomonas aureofaciens NB-1 and Pseudomonas fluorescens 8573 strains adequately the properties of organic solvents by means of the multiparameter linear energy equations. the it was established that the basicity of the solvents is the main characteristic, that effects on the process extraction of glycolipids from cultural liquids of both strains. It was shown that alcohols and esters are rational extracting agents for the glycolipids, which can be explained, in the case of esters, by the solvation of the ester group and, in the case of alcohols, due to the formation of hydrogen bonds. This effect is related to the parallel position of the alkyl chains of alcohol and β-oxidecanoic acid of the glycolipid molecule. The obtained results of carried out research have a great importance for the correct selection of the optimal extracting agents for obtaining of new perspective biotechnological products.