Comprehensive computational analysis of chromosome 11

Abstract

Even before the onset of Human Genome Project in 1990, various research, analysis and experiments was going on human genome. In human genome, approximately 3 billion base pairs are structured in the form of 23 pairs of chromosomes. Chromosome 11 is a chromosome that is rich in disease and has a size of 134 million base pairs. From over 1000 genome web servers, the sequence data for the chromosome 11 has been taken as a (.bam) file. On Chromosome 11, approximately 45 different analyses were performed across various fields and categories, such as determining the sequence quality, over represented sequence, peak model, visualizing BAC end pairs, secondary structure prediction by analyses hydroxyl cleavage sites, GC percentage, studying phenotype and disease associated with respect to chromosome 11. Here, we have analysed the evolutionary relationship by isolating positively selected genes across 6 species; predicted the t-RNA and RNA secondary structures and aligned them discretely with the human genome chromosome 11. All these analyses was done primarily with the help of two tools, 1) Web based program-NEBULA and 2) UCSC genome browser.