Shock Wave Interaction with Micro-Filament Network in a Cancer Ce1l line

Abstract

Interaction of shock waves with renal tumor cell line (ACHN) has been studied with regard to their membrane micro-filament structure as a major cellular system to resist mechanical stress. ACHN monolayer cultured on a cover slide glass was treated with 16 MPa peak pressure focused underwater shock waves. The morphological deformations were found to be associated with disorganization of the intracellular cytoskeletal filaments. In order to simulate the interaction of shock waves with fibrillar network structure of cells, thin porous layers of cotton immersed in water were exposed to underwater shock waves. Attenuation of shock over-pressures were measured with needle and fiber optic probe hydrophones. The motion of shock waves was quantitatively visualized. The experimental results were extended to understand the complex process of shock/cell interactions, which would happen during ESW cancer therapy