Freeze-Dried Matrix as an Alternative to Solution Mixing for Enzyme Analysis in a Micro Flow Channel

Abstract

A freeze-dried matrix packed in a micro flow channel was used to analyze an enzymatic reaction rapidly without using external pumps. The very porous and fine precipitated matrix facilitated the permeation of a sample solution into the flow channel and the dissolution of the components. The amount of products produced as a result of the enzymatic reactions was sufficient and a significant increase in the resulting sensor output was observed. The applicability of the device was demonstrated in the determination of the activities of enzymes, the analysis of enzyme kinetics, and the monitoring of a multi-step reaction. This can be a basic technology in conducting microfluidic diagnostic systems with higher functions including the analysis of enzymatic reactions.