Detection of a Macromolecule Denaturation With Microwave Dielectric Spectroscopy based on Hydration Modifications

K. Grenier, G. Pratviel, Hugo Mazur, D. Dubuc
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Abstract

This paper focuses on demonstrating the possible detection with microwave dielectric spectroscopy of the hydration modification of a protein submitted or not to a chaotropic agent. The case study of the denaturation of the large BSA protein with urea is investigated. A hydration contrast is extracted from microwave measurements and presents a linear relationship with the concentration of the denaturing agent. This result demonstrates that microwave dielectric spectroscopy could contribute to evaluate intramolecular change of conformation (structuration/destructuration) of biomacromolecules based on hydration modifications.
基于水化改性的微波介电光谱检测大分子变性
本文重点介绍了微波介电光谱法检测蛋白质在加入或未加入混沌剂的情况下水化改性的可能性。研究了尿素对大BSA蛋白变性的影响。从微波测量中提取水化对比,并与变性剂浓度呈线性关系。这一结果表明,微波介电光谱有助于评价基于水合修饰的生物大分子分子内构象(结构/破坏)的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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