The Optimum Elution Temperature and Time in Absorption-Elution Test Using Commercially Available Monoclonal Antibodies for ABO Blood-Typing from Hair Samples

Abstract

The optimum elution temperature and optimum elution time of absorption-elution test for ABO blood-typing from hair samples using commercially available monoclonal antibody were examined. Elution temperature was examined in the range of 51-57°C and elution time was examined in the range of 2-15 min. The eluting antibodies were semi-quantitatively analyzed by ELISA using synthetic blood group antigens. The optimum elution temperature and optimum elution time were determined for each monoclonal antibody. The procedure of absorption-elution method for hair samples was improved and the improved procedure was evaluated by a blind test. The improved procedure of the absorption-elution test gave a satisfactory result.