Regeneration of Cartilage Tissue by Comparing the Natural and Synthetic Biomaterials during ADSCs Differentiation

Abstract

Objectives: Human adipose-derived stem cells have shown chondrogenic differentiation for cartilage engineering. In the present study, we evaluated different scaffolds for chondrogenic differentiation of Human adipose-derived stem cells. Methods: cells were cultured and chondrogenic differentiation was investigated in Polycaprolactone, Platelet Rich Plasma, and Fibrin glue scaffolds by MTT, Real time and histology. Results: Cell proliferation and collagen levels in Platelet Rich Plasma scaffolds were significantly higher than other scaffolds. The levels of Sox9 and col II were up regulated in Platelet Rich Plasma. Histological examination revealed that the pores of the scaffolds were filled cells. Conclusion: The results show that 3D Platelet Rich Plasma scaffolds provide a better environment for chondrogenic differentiation than others.