Assessing the Contamination of the External Surfaces of Reused Calen® System Tubes

L. F. D. A. Figueiredo, C. Falcāo, N. S. Rocha, E. C. P. Junior, Márcia Maria Negreiros Pinto Rocha, P. Jacques
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Abstract

Objective: To determine the presence of contamination on the external surface of reused Calen® System tubes before (TA) and after (TB) the action of four disinfectants: 0.12% chlorhexidine, 2% glutaraldehyde, 70 % alcohol and 1% sodium hypochlorite. Material and Methods: Microbiological collection of 20 tubes from dental clinics was performed with the aid of sterile swabs moistened in saline and rubbed for one minute on their surfaces and inserted in tubes containing 4 ml BHI and incubated under microaerophilic conditions (37°C for 24h) for further Mc Farland (F0.5 to F10) and Gram staining analyses. For disinfection (TB), cotton wicks with 1 ml of disinfectant or distilled water (control) was rubbed for 30 seconds, and after drying by evaporation, new samples were collected from the surfaces, which were submitted to the same incubation and Gram staining process for the analyses of slides. Results: Prevalence of F4 and F5 in the same proportion in TA and F1 in TB. Gram + cocci were present in 100% of TA and TB, and prevalence of Gram + cocci, Gram + and – bacilli, spores, Gram - cocci and vibrios in descending order. Conclusion: All external surfaces of reused Calen® System tubes were contaminated before and after disinfection. In descending order, the disinfectant that provided a greater reduction in the Mc Farland scale and thus a greater reduction in the number of microorganisms was 1% sodium hypochlorite, followed by 2% glutaraldehyde, 0.12% chlorhexidine, 70% alcohol and distilled water (control).
评估重复使用的Calen®系统管的外表面污染
目的:测定0.12%氯己定、2%戊二醛、70%酒精和1%次氯酸钠4种消毒剂对重复使用的Calen®系统试管外表面的污染情况。材料和方法:从牙科诊所收集20根试管,无菌拭子用生理盐水湿润,在试管表面摩擦1分钟,然后插入含有4ml BHI的试管中,在微气条件下(37°C孵育24小时)进行进一步的Mc Farland (F0.5至F10)和革兰氏染色分析。对于消毒(TB),用含1 ml消毒剂或蒸馏水(对照)的棉芯摩擦30秒,蒸发干燥后,从表面收集新样品,将其提交到相同的培养和革兰氏染色过程中进行载玻片分析。结果:结核中F4和F5的患病率与结核中F1的患病率相同。结核杆菌和结核杆菌100%检出革兰氏球菌,革兰氏球菌、革兰氏和-革兰氏杆菌、孢子、革兰氏球菌和弧菌的检出率依次为低至低。结论:重复使用的Calen®系统试管消毒前后表面均有污染。从降序上看,减少麦氏法兰德氏规模和减少微生物数量最多的消毒剂是1%次氯酸钠,其次是2%戊二醛、0.12%氯己定、70%酒精和蒸馏水(对照)。
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