POTENTIAL IN BIOETHANOL PRODUCTION FROM VARIOUS AGRO WASTES FERMENTING BY MICROORGANISMS USING CARROT PEEL, ONION PEEL, POTATO PEEL AND SUGAR BEET PEEL AS SUBSTRATES.

Fungal Territory Pub Date : 2021-04-02 DOI:10.36547/FT.132
Dr.isaie Mushimiyimana, F. Niyitanga, Celestin Sirimu
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Abstract

Large amount of agro wastes are produced in Rwanda each year. The global annual potential bioethanol production from the major vegetables wastes such as carrot peel, onion peel, potato peel and sugar beet peel was estimated. Those wastes processing were successfully used as raw materials for the production of bioethanol, employing by cellulase produced from various filamentous fungi including Cladosporium cladosporioides was used for hydrolysis and the fermentation of the hydrolyzed samples was done using Sacharomyces cerevisiae. The fermented product was purified by primary distillation process at 79 °C and the fraction was collected. The ethanol is then determined by specific dichromate method and Gas Chromatography. Instantaneous saccharification and fermentation process yielded maximum ethanol in the substrate of carrot peel was 16.9 % at 21st day and further confirmed by Gas chromatography and the yield of ethanol obtained was 15.8 %.
以胡萝卜皮、洋葱皮、土豆皮和甜菜皮为底物,利用微生物发酵各种农业废弃物生产生物乙醇的潜力。
卢旺达每年产生大量的农业废物。估计了全球每年利用胡萝卜皮、洋葱皮、土豆皮和甜菜皮等主要蔬菜废弃物生产生物乙醇的潜力。利用包括枝孢枝孢霉(Cladosporium cladosporioides)在内的多种丝状真菌产生的纤维素酶进行水解,并利用酿酒酵母(Sacharomyces cerevisiae)对水解后的样品进行发酵,成功地将这些废弃物作为生产生物乙醇的原料。在79℃条件下对发酵产物进行一次精馏纯化,并收集馏分。然后用特定重铬酸盐法和气相色谱法测定乙醇。瞬时糖化发酵工艺在胡萝卜皮底物中的乙醇产量在第21天最高为16.9%,经气相色谱进一步证实乙醇产量为15.8%。
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