Light Scattering Microscopy for Tumor Cell Recognition

Abstract

Light scattering microscopy is suggested to providesome insight into cell architecture and to giveinformation on cell malignancy or morphologicalchanges upon necrosis or apoptosis. In the presentpaper a microscope setup for scattering experimentswith high angular resolution is reported, and firstdata obtained from test models (polystyrene beads),cell monolayers and 3-dimensional cell spheroidsare reported. Data are compared with simulationsbased on Mie scattering and discussed in view oftheir possible use for tumor cell recognition withintissue samples. The discussion includes presentlimitations due to spatial resolution or inhomogeneous illumination and suggests possibilities ofimprovement.