Utilization of Vermiwash for the Production of Liquid Biofertilizers and Its Effect on Viability of Inoculant Bacteria and Green Bean Germination

N. Arfarita, M. Lestari, C. Prayogo
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引用次数: 3

Abstract

Contribution of biofertilizer in Indonesia as an alternative material for improving soil fertility and crop production is still limited. This may due to several major obstacles on its utilization and effectiveness regarding to it quality in term of its microbial viability of inoculants on formulations. This study aims to determine the effect of liquid biofertilizer formulations made from vermiwash carrier material on indigenous bacterial viability and its testing on germination of green beans ‘Vima-1’. The study included: (1) optimization of pH for the growth of inoculants bacterial consortium in vermiwash formulations with various additives, (2) viability tests and (3) pathogenicity tests of biological fertilizers on green bean sprouts (vegetative phase). The results showed that the best pH for the growth of the consortium of three bacterial isolates was at 5.5. Liquid formulation with a carrier material in the form of vermiwash plus 1% PEG (VP3 treatment) supported the highest viability of the bacterial consortium during 8 weeks incubation. All treatments did not inhibit the growth of green bean sprouts and symptom of pathogenicity was absent in all treated plants. From all treatments, the tallest green bean sprout was detected at VP3 treatment.
蚯蚓水在液体生物肥料生产中的应用及其对接种菌活力和绿豆发芽的影响
在印度尼西亚,生物肥料作为提高土壤肥力和作物产量的替代材料的贡献仍然有限。这可能是由于其利用和有效性方面的几个主要障碍,就其质量而言,接种剂在配方上的微生物活力。本研究旨在研究以蚯蚓为载体的液体生物肥料配方对青豆“Vima-1”原生细菌活力的影响及其发芽试验。研究内容包括:(1)优化不同添加剂接种菌群在蚯蚓水配方中生长的pH值;(2)生物肥料对绿豆芽(营养期)的生存力试验;(3)生物肥料对绿豆芽的致病性试验。结果表明,3株菌群的最佳生长pH为5.5。在8周的孵育期间,以蠕虫水形式的载体材料加1% PEG (VP3处理)的液体制剂支持细菌联合体的最高活力。所有处理均未抑制绿豆芽的生长,所有处理植株均无致病性症状。在所有处理中,VP3处理的绿豆芽最高。
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