Luteolytic effect of the antiprogestin and antiglucocorticoid agent RU486 in rats

Journal of steroid biochemistry Pub Date : 1990-08-14 DOI:10.1016/0022-4731(90)90091-6

Satoko Arakawa, Akira Kambegawa, Shoichi Okinaga, Kiyoshi Arai

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引用次数: 9

Abstract

Ovarian cells of pregnant rats were cultured with synthetic progestins (R5020, R2323), dexamethasone and RU486. Progesterone and 20α-hydroxy-pregn-4-en-3-one (20α-dihydroprogesterone) in the medium were measured by specific radioimmunoassay. Both R5020 and R2323 increased concentrations of these intrinsic progestins. RU486 decreased concentrations of progesterone, however, the addition of R5020 or R2323 counteracted this action.

Immature hypophysectomized rats treated with pregnant mare serum gonadotropin (PMS) and human chorionic gonadotropin (hCG) were administered with RU486; the serum levels of progesterone and 20α-dihydroprogesterone tended to decrease.

R5020 and R2323 inhibited the effect of 3β -hydroxy steroid dehydrogenase (3β-HSD), whereas RU486 did not. Inhibition of the cholesterol side chain cleavage enzyme (CSCC) by RU486 was more marked than that by R5020 or R2323.

These results show that RU486 decreases progesterone synthesis in cultured ovarian cells. A part of the mechanism may involve an inhibition of CSCC.

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抗黄体酮和抗糖皮质激素RU486对大鼠的溶血作用

用合成孕激素(R5020、R2323)、地塞米松和RU486培养妊娠大鼠卵巢细胞。用特异性放射免疫法测定培养基中的孕酮和20α-羟基孕酮(20α-二氢孕酮)含量。R5020和R2323均增加了这些内在孕激素的浓度。R5020或R2323的加入抵消了RU486降低孕酮浓度的作用。用妊娠母马血清促性腺激素(PMS)和人绒毛膜促性腺激素(hCG)治疗未成熟垂体切除大鼠，给予RU486;血清孕酮和20α-二氢孕酮水平有降低的趋势。R5020和R2323抑制了3β-羟基类固醇脱氢酶(3β- hsd)的作用，而RU486则无此作用。与R5020和R2323相比，RU486对胆固醇侧链切割酶(CSCC)的抑制作用更为明显。上述结果表明，RU486可降低体外培养卵巢细胞的孕酮合成。该机制的一部分可能涉及对CSCC的抑制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of steroid biochemistry

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