PEGDA-based photocrosslinking platform for real time cell trapping

Abstract

Poly (ethylene glycol)-diacrylate (PEG-DA) is a commonly used material in tissue engineering. It is cross-linking via UV-initiated photopolymerization to form the PEG-DA microstructures. This paper reports the fabrication of capillary PEG-DA architectures on photopolymerizable hydrogel characteristic. In the UV-initiated photopolymerization, oxygen can damage the photopolymerization reaction that to from microinterstices. In PDMS-based chip, a little oxygen in PDMS material is stored in PEG-DA material. By the way, we designed PEGDA-based V-shape microbarriers to trapped HMEC-1 cell for another biotechnology. For V-shaped microbarriers, we used the solution of the PEG-DA had been exposed to form V-shaped barriers by mask pattern. Cell trapping array platform was used for understanding fundamental cell studies which are cell-cell interactions and cell responses, such as hydrodynamic trapping system.