Protein engineering for natural product biosynthesis: expanding diversity for therapeutic applications

Sarah Oluwatobi Otun, J. A. Lerma-Escalera, Khayalethu Ntushelo, Ikechukwu Achilonu
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Abstract

In this dispensation of the fourth industrial revolution, protein engineering has become a popular approach for increasing enzymatic activity, stability, and titer in the biosynthesis of natural products. This is attributed to its numerous advantages (over direct isolation from plants or via chemical synthesis), including decreasing or eliminating reaction byproducts, high precision, moderate handling of intricate and chemically unstable chemicals, overall reusability, and cost efficiency. Recently, protein engineering tools have advanced to redesign and enhance natural product biosynthesis. These methods include direct evolution, substrate engineering, medium engineering, enzyme engineering and immobilization, structure-assisted protein engineering, and advanced computational. Recent successes in implementing these emerging protein engineering technologies were critically discussed in this article. Also, the advantages, limitations, and applications in industrial and medical biotechnology were discussed. Last, future research directions and potential were also highlighted.
天然产物生物合成的蛋白质工程:扩大治疗应用的多样性
在这个第四次工业革命的时代,蛋白质工程已经成为提高天然产物生物合成中酶活性、稳定性和滴度的一种流行方法。这归功于其众多优势(与直接从植物中分离或通过化学合成相比),包括减少或消除反应副产物,高精度,对复杂和化学不稳定化学品的适度处理,整体可重用性和成本效益。近年来,蛋白质工程工具已经发展到重新设计和增强天然产物的生物合成。这些方法包括直接进化、底物工程、培养基工程、酶工程和固定化、结构辅助蛋白质工程和高级计算。本文对最近在实施这些新兴蛋白质工程技术方面取得的成功进行了批判性的讨论。此外,还讨论了其优点、局限性及其在工业和医学生物技术中的应用。最后,对未来的研究方向和潜力进行了展望。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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