KERAGAMAN GENETIK SEMAI TIMOHO (Kleinhovia hospitaLinn.) BERDASARKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA (RAPD)

JURNAL PEMULIAAN TANAMAN HUTAN Pub Date : 2021-12-30 DOI:10.20886/jpth.2021.15.2.85-93

I. Nurtjahjaningsih, L. Hakim, Y. Hutomo, A. Rimbawanto, Aypbc Widyatmoko, I. Prihatini, Maryatul Qiptiyah, P. Sulistyawati, Wahyunisari Wahyunisari

{"title":"KERAGAMAN GENETIK SEMAI TIMOHO (Kleinhovia hospitaLinn.) BERDASARKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA (RAPD)","authors":"I. Nurtjahjaningsih, L. Hakim, Y. Hutomo, A. Rimbawanto, Aypbc Widyatmoko, I. Prihatini, Maryatul Qiptiyah, P. Sulistyawati, Wahyunisari Wahyunisari","doi":"10.20886/jpth.2021.15.2.85-93","DOIUrl":null,"url":null,"abstract":"Timoho (Kleinhovia hospita Linn.) has high economic value for medicine and construction. However, information on genetic diversity and conservation genetic has been limited by the lack of genetic data. This study aimed to assess genetic diversity of timoho seedling using RAPD markers. Leaf samples of seedlings were collected from a nursery at CFBTI in Yogyakarta; the seedlings originated from plantation in Gunungkidul (GK) and arboretum in Faculty of Forestry, GMU (FK). Seven out of 22 screened RAPD markers were stable in amplification and consisted of 61 polymorphic loci; then these markers were used to analyse genetic diversity and population structure of 48 seedlings from GK and FK. The genetic diversity and structure parameters were calculated by GenAlex software. A moderate level of genetic diversity (mean uHE=0,326) and great genetic distance between the two populations (DA=0.119) were found in this species. PCoA analysis showed that the 48 individuals were divided into two clusters according to their originated population. Appropriate conservation strategies were proposed to protect genetic diversity of this species","PeriodicalId":239445,"journal":{"name":"JURNAL PEMULIAAN TANAMAN HUTAN","volume":"182 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"JURNAL PEMULIAAN TANAMAN HUTAN","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20886/jpth.2021.15.2.85-93","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Timoho (Kleinhovia hospita Linn.) has high economic value for medicine and construction. However, information on genetic diversity and conservation genetic has been limited by the lack of genetic data. This study aimed to assess genetic diversity of timoho seedling using RAPD markers. Leaf samples of seedlings were collected from a nursery at CFBTI in Yogyakarta; the seedlings originated from plantation in Gunungkidul (GK) and arboretum in Faculty of Forestry, GMU (FK). Seven out of 22 screened RAPD markers were stable in amplification and consisted of 61 polymorphic loci; then these markers were used to analyse genetic diversity and population structure of 48 seedlings from GK and FK. The genetic diversity and structure parameters were calculated by GenAlex software. A moderate level of genetic diversity (mean uHE=0,326) and great genetic distance between the two populations (DA=0.119) were found in this species. PCoA analysis showed that the 48 individuals were divided into two clusters according to their originated population. Appropriate conservation strategies were proposed to protect genetic diversity of this species

查看原文本刊更多论文

Timoho (Kleinhovia hospital Linn.)具有很高的药用和建筑经济价值。然而，由于缺乏遗传数据，有关遗传多样性和保护遗传的信息一直受到限制。本研究旨在利用RAPD标记评价铁莫幼苗的遗传多样性。从日惹CFBTI的苗圃收集幼苗叶片样本;幼苗产自Gunungkidul (GK)人工林和GMU (FK)林学院植物园。筛选的22个RAPD标记中有7个扩增稳定，共61个多态性位点;然后利用这些标记分析了GK和FK的48株幼苗的遗传多样性和群体结构。利用GenAlex软件计算遗传多样性和结构参数。遗传多样性中等(平均uHE= 0.326)，居群间遗传距离较大(DA=0.119)。PCoA分析结果表明，48个个体根据其原居群划分为两个聚类。提出了适当的保护策略，以保护该物种的遗传多样性

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

JURNAL PEMULIAAN TANAMAN HUTAN

自引率

0.00%

发文量