Using an in vitro Blood-Brain Barrier Model to Characterize Magnetic Nanoparticle Permeability

Abstract

In the present study, using murine brain endothelioma cells, an in vitro blood-brain barrier model was developed and confirmed. Confirmation of the blood-brain barrier model was completed by examining the permeability of FITC-Dextran at increasing exposure times in serum-free medium and comparing such values with values in the literature. After such confirmation, the permeability of five different nanoparticle samples was determined by this blood-brain barrier model. Through such experiments, magnetic nanoparticles suitable for MRI use which would not pass through the blood brain barrier and magnetic nanoparticles suitable for drug delivery to the brain which would pass through the blood brain model were identified.