Development of ion-channel based biosensors using polymerizable lipids

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society Pub Date : 1988-11-01 DOI:10.1109/IEMBS.1988.95050

P. Yager, J. Person, K. Seib

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引用次数: 1

Abstract

As a step in developing the potential of polymerizable lipids for stabilization of bilayers for biosensor applications, the authors have demonstrated that asymmetric bilayers consisting of one monolayer of polymerized diacetylenic lecithin and the other monolayer consisting of asolectin can support the function of alamethicin, although this function is somewhat modified. Single monolayers of 1, 2-bis- (10, 12-tricosadiynoyl)-sin-glycero- 3-phosphocholine (DC/sub 23/PC) were formed at the air-water interface and polymerized with a low-pressure mercury lamp. Other monolayers were formed from asolectin, and a patch electrode was passed through them to form an asymmetric bilayer. Membrane stabilization in the form of a substantial increase in the bilayer breakdown voltage was seen. When the ion-channel-forming peptide alamethicin was presented to the asolectin monolayer it formed channels through such bilayers, although the turn-on voltage for the channels was substantially higher and the conductivity lower than for symmetric asolectin bilayers.<>

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可聚合脂质离子通道生物传感器的研制

作为开发可聚合脂质用于生物传感器应用的双分子层稳定的潜力的一步，作者已经证明，由一层聚合的二乙基卵磷脂和另一层由聚乳酸素组成的不对称双分子层可以支持alamethicin的功能，尽管这种功能有所修改。在空气-水界面处形成了1,2 -二-(10,12 -三osadiynoyl)-辛-甘油- 3-磷酸胆碱(DC/sub 23/PC)单分子层，并在低压汞灯下进行了聚合。其他的单分子层是由一种凝集素形成的，贴片电极穿过它们形成不对称的双分子层。膜稳定的形式是双层击穿电压的大幅增加。当形成离子通道的肽alamethicin被呈现给偶联素单层时，它通过这些双层形成通道，尽管这些通道的导通电压比对称偶联素双层高得多，电导率低于对称偶联素双层

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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