A Centrifugal Chemiluminescence Detection Platform and Its Application for Nucleic Acid Virus (H7N9)

Abstract

Chemiluminescence detection has been used to determine the concentration of nucleic acid virus. At present, chemiluminescence detection is mostly a large, immovable chemiluminescence detection instrument, and some steps to extract supernatant still need to be completed manually. To solve this problem, a centrifugal chemiluminescence detection system was established. The CMOS detector with high sensitivity and small volume was combined with centrifugal force micro-fluidic platform to realize the integration process of sample reaction and detection. The system has been successfully applied to the detection of nucleic acid virus (H7N9). The detection process of nucleic acid virus (H7N9) completed in the original laboratory was designed on a microfluidic chip. The flow direction of liquid is driven by centrifugal force platform, and the whole reaction process is controlled by logic components on the chip. Finally, the luminous intensity is detected by CMOS detector.