Sleeve Gastrectomy Ameliorates Mrna Expression of MatrixMetalloprotease I, III, V, IL-1a and IL-6 Genes in Substantia Nigra Tissuesof Obese Rats

Abstract

Aim: To reveal metalloproteinase (MMP)-1, MMP-3, MMP-5, interleukin (IL)-1a and IL-6 gene expressions in substantia nigra region of brain in rats which undergone sleeve gastrectomy (SG) surgery. Methods: Rats were allocated into three groups in random, which were normal rats (Group I) (n=14), obese rats (Group II) (n=14), and obese rats subjected to SG (Group III) (n=14). MMP-1, MMP-3, MMP-5, IL-1a and IL-6 gene expressions were determined by polymerase chain reaction (PCR) and Real-time polymerase chain reaction (RTqPCR). Results: When normal (Group I), and obese (Group II) rats were compared, a decrease in expressions of MMP- 3 and IL-6 genes was observed in Group II. When obese rats (Group II) and obese rats subjected to SG (Group III) were compared, increases in the expressions of MMP-3 and IL-6 genes were observed in Group III. This phenomenon demonstrates that SG decreases obesity and consequently increases expressions of MMP-3 and IL-6 genes. Conclusion: These data show alterations of MMP-3 and IL-6 genes in the substantia nigra tissue of obese rats, consistent with the possibility that these changes may contribute to disease molecular background.