Isolation and Screening of Potential Cellulase Free-Xylanase Producing Bacterial Strains from Cow Dung

Abstract

Research on xylanase has markedly increased due to its potential applications in pulping and bleaching processes using cellulose free preparations, textile processes, enzymatic saccharification of lignocellulosic materials and waste treatment. The objective of present study was to isolate and screen potential microbes from cow dung with respect to cellulase free xylanase production. Twelve isolates were obtained from cow dung with different frequencies of occurrence. Among the different culture isolates, the selection of selective isolate was done depending upon the clear zone formation on xylan agar medium. Submerged fermentation was done with the selective isolates to identify the cellulase free xylanase stains at pH 7.0 and at temperature 37°C. Bacillus sp. X6 was capable of producing xylanase of 15.32 IU/ml using basal salt medium under submerged fermentation